Folia Microbiologica

, Volume 27, Issue 2, pp 131–137 | Cite as

Effects of nutrients present in Bold’s basal medium on the green algaStigeoclonium pascheri

  • S. C. Agrawal
  • Y. S. R. K. Sarma


The effects of varying concentrations of nutrients present in Bold’s basal medium on the extent of colony formation from vegetative fragments, sporulation and spore germination of the green algaStigeoclonium poscheri were studied. A decrease of colony formation was observed in media deficient in MgSO4, NaNO3, phosphates, and containing a 10-fold increase of H3BO3. Sporulation decreased in the same media. However, sporulation was greater in an increasing order in media containing 2- to 10-fold increase in MgSO4. There was a decrease in spore germination in media deficient in phosphates, MgSO4, containing 5- or 10-fold MgSO4, or containing 2- to 10-fold of CaCl2, H3BO3 or microelements. Spore germination increased in media containing 2-fold MgSO4, deficient in H3BO3 or microelements or containing none of the three micronutrient solutions.


Basal Medium Colony Formation Spore Germination Vegetative Fragment Colony Appearance 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1982

Authors and Affiliations

  • S. C. Agrawal
    • 1
  • Y. S. R. K. Sarma
    • 1
  1. 1.Department of BotanyBanaras Hindu UniversityVaranasiIndia

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