Folia Microbiologica

, Volume 27, Issue 2, pp 87–92 | Cite as

Srinivasan’s coagulation test in taxonomically classified streptomycetes

  • A. Řičicová
  • L. Pokorná
  • P. Sajdl


Srinivasan’s coagulation test was performed in 18 strains of the genusStreptomyces and one strain of the genusActinoplanes. The highest coagulation activity was detected in strains systematically classified in a series of streptomycetes with pink or red aerial mycelium:S. erythreus, Streptomyces sp. AJ/22,S. roseo-luteus andS. griseofuscus. With the exception ofS. griseofuscus these three cultures also exhibited the highest inhibitory activity againstB. subtilis. When using hemoglobin as substrate it was possible to detect acid, neutral and alkaline proteinases with the highest proteolytic activity at pH 3.0 to 4.0 in the most active strain ofS. erythreus.


Streptomyces Alkaline Proteinase Aerial Mycelium Coagulation Activity High Inhibitory Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bergey’s Manual of Determinative Bacteriology, 8th ed. (R.E. Buchanan, N.E. Gibbons, Eds). Williams & Wilkins, Baltimore 1974.Google Scholar
  2. Bessell Ch.J., Poges S., Anslow W.K., Mortimer A.M., Oak S., Fletcher D.L., Rhodes A.: Antibiotic production.US Pat. 3 013947 (1961).Google Scholar
  3. Krasilnikov N.A.: Actinomycetes—antagonists and antibiotic compounds. (In Czech) Publ.House Czech.Acad.Sci., Prague 1953.Google Scholar
  4. Pridham T.G., Hesseltine C.W., Benedict R.G.: A guide for the classification ofStreptomycetes according to selected groups.Appl. Microbiol.6, 52 (1958).PubMedGoogle Scholar
  5. Řičicová A., Podojil M.: Quantitative assay of granaticin by the microbiological diffusion method.Folia Microbiol.10, 299 (1965).CrossRefGoogle Scholar
  6. Řičicová A., Hodinář F., Řeháček Z.: Characterization and comparative studies ofStreptomyces sp. 246 producing a cytostatic antibiotic.Folia. Microbiol.14, 40 (1969).CrossRefGoogle Scholar
  7. Řičicová A., Pokorný V., Řeháček Z.: A psychrophilic strainActinoplanes sp. 220.Folia Microbiol.24, 462 (1979).Google Scholar
  8. Shirling E. B., Gottlieb D.: Methods for characterization ofStreptomyces species.Internat. J. Syst. Bacteriol.16, 313 (1966).Google Scholar
  9. Shirling E.B., Gottlieb D.: Cooperative description of type cultures ofStreptomyces. III. Additional species descriptions from first and second studies.Internat. J. Syst. Bacteriol.18, 279 (1968).CrossRefGoogle Scholar
  10. Shirling E.B., Gottlieb D.: Cooperative description of type strains ofStreptomyces. V. Additional descriptions.Internat. J. Syst. Bacteriol.22, 265 (1972).Google Scholar
  11. Ševčík V., Doležilová L., Řeháěk Z., Musílek V., Vaněk Z.: Laboratory fermentation of erythromycin. (In Czech) Final report. Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 1957.Google Scholar
  12. Ševčík V., Podojil M., Řičicová A.: Production of actinomycin B by a new strain ofStreptomyces antibioticus.Folia Biol. (Prague)4, 328 (1958).Google Scholar
  13. Turková J., Mikeš O., Gančev K., Boublík M.: Isolation and characterization of alkaline proteinase ofAspergillus flavus.Biochim. Biophys. Acta173, 100 (1969).Google Scholar
  14. Waksman S.A.:The actinomycetes. Vol. I. Williams & Wilkins, Baltimore 1959.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1982

Authors and Affiliations

  • A. Řičicová
    • 1
  • L. Pokorná
    • 2
  • P. Sajdl
    • 1
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4
  2. 2.Dairy IndustryResearch and Development PlantPrague 6

Personalised recommendations