Folia Microbiologica

, Volume 27, Issue 2, pp 76–80 | Cite as

Relationship between the composition of phospholipids and respiratory activity of choline-deficient mutants ofNeurospora crassa

  • H. Fečíoková
  • Y. Gbelská
  • Z. Baráth
  • M. Grmanová


Phosphatidylcholine is one of the most frequent phospholipid components of the inner mitochondrial membrane ofNeurospora crassa. Quantitative analysis of phospholipids of the wild strain ofNeurospora crassa and of its twocho mutants showed that these strains did not significantly differ in the content of phosphatidylcholine. Mutants cultivated in a medium without choline contained, as compared with the wild strain, an increased amount of phosphatidylserine and a decreased quantity of phosphatidic acid. Respiratory activity increased and sensitivity to inhibitors of respiration changed. It is likely that the presence of choline in the growth medium exerts a regulatory effect on the cell metabolism of these mutants.


Choline Ethanolamine Respiratory Activity Phosphatidic Acid Hydroxamic Acid 
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phosphatidic acid










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  1. Bruni A., Racker E.: Resolution and reconstitution of the mitochondrial electron transport system.J. Biol. Chem. 243, 962 (1968).PubMedGoogle Scholar
  2. Drabikowska A.K.: Oxidation processes and ubiquinone localization in the branched respiratory system ofmi-1 mutant ofNeurospora crassa.Acta Biochim. Polon. 22, 169 (1975).PubMedGoogle Scholar
  3. Juretič D.: The effect of phosphatidylcholine depletion on biochemical and physical properties of aNeurospora crassa membrane mutant.Biochim. Biophys. Acta 469, 137 (1977).PubMedCrossRefGoogle Scholar
  4. Kagawa Y., Kandrach A., Racker E.: Partial resolution of the enzymes catalyzing oxidative phosphorylation.J. Biol. Chem. 248, 676 (1973).PubMedGoogle Scholar
  5. Kimelberg H.K., Lee C.P., Claude A., Mrena E.: Interaction of cytochrome c with phospholipid membranes.J. Membr. Biol. 2, 235 (1970).CrossRefGoogle Scholar
  6. Lambowitz A.M., Slayman C.W.: Cyanide-resistant respiration inNeurospora crassa.J. Bacteriol. 108, 1087 (1971).PubMedGoogle Scholar
  7. Lowry O.H., Rosenbrough N.J., Farr A.L., Randall R.J.: Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265 (1951).PubMedGoogle Scholar
  8. Rouser G., Fleischer S., Yamamoto A.: Two dimensional thin-layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots.Lipide 5, 494 (1970).CrossRefGoogle Scholar
  9. Rydström J.: Energy-linked nicotinamide nucleotide transhydrogenases.Biochim. Biophys. Acta 463, 151 (1977).Google Scholar
  10. Scarborrough G.A., Nyc J.F.: Methylation of ethanolamine phosphatides by microsomes from normal and nutrient strains ofNeurospora crassa.J. Biol. Chem. 242, 238 (1967).Google Scholar
  11. Šubík J.: Conditions of isolation of lipids from yeast.Chem. Zvesti 23, 616 (1969).Google Scholar
  12. Tzagaloff A., Mac Lennen, D.H.: Studies of the electron-transfer system.Biochim. Biophys. Acta 99 476 (1965).Google Scholar
  13. Weete J.D.:Fungal Lipid Biochemistry, Vol. 1 p. 239. Plenum Press, New York-London 1974.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1982

Authors and Affiliations

  • H. Fečíoková
    • 1
  • Y. Gbelská
    • 2
  • Z. Baráth
    • 1
  • M. Grmanová
    • 1
  1. 1.Biochemical InstituteKomenský UniversityBratislavaCzechoslovakia
  2. 2.Research Institute of Food TechnologyBratislavaCzechoslovakia

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