Comparative analysis of the pig BAC sequence involved in the regulation of myostatin gene
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Myostatin (GDF8, MSTN) is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass. In order to study its expression and regulatory mechanism deeply, we have presented a comparative analysis of about 170-kb pig BAC sequence containing the myostatin gene among pig, human and mouse. The genomic region is characterized by high interspersed repeats and low G+C content. As for the myostatin gene, a higher sequence similarity is found between human and pig than between these species and the mouse. One striking feature is that the structure of two TATA-boxes in the nearby downstream of CCAAT-box is identified in the promoter. Further analysis reveals that the TATA-box1 is responsible for the transcription in pig and human, but the TATA-box2 acts on the transcription in mouse. The other interesting feature is that two polyadenylation signal sequences (AATAAA) exist in 3’UTR of the pig myostatin gene. Moreover, a large number of potential transcription factor-binding sites are also identified in evolutionary conserved regions (ECRs), which may be associated with the regulation of myostatin. Many putative transcription factors play an important role in the muscle development, and the complex interaction between myostatin and these factors may be required for proper muscle development.
Keywordsmyostatin comparative analysis transcriptional factors muscle development
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- 8.Strail, A., Kopecny, M., Genomic organization, sequence and polymorphism of the porcine myostatin (GDF8;MSTN) gene, Animal Genetics, 1999, 30(6): 468–470.Google Scholar
- 24.Morishita, R., Gibbons, G. H., Horiuchi, M., Kaneda, Y., Ogihara, T., Dzau, V. J., Role of AP-1 complex in angiotensin II-mediated transforming growth factor-beta expression and growth of smooth muscle cells: Using decoy approach against AP-1 binding site, Biochem. Biophys. Res. Commun., 1998, 243(2): 361–367.CrossRefGoogle Scholar
- 39.Shibata, H., Yoda, Y., Kato, R. et al., A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with U2afbp-rs gene: An association with a short tandem repeat and hypermethylated region, Genomics, 1998, 4930–4937.Google Scholar
- 47.Dias, P., Dilling, M., Houghton, P., The molecular basis of skeletal muscle differentiation, Semin. Diagn. Pathol., 1994, 11(1): 314.Google Scholar