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Biologia Plantarum

, 38:153 | Cite as

Polyamines and senescence of maintenance foliage of tea,Camellia sinensis L.

  • R. K. Kakkar
  • P. K. Nagar
Brief Communication

Abstract

In leaf discs of maintenance foliage of tea (Camellia sinensis) polyamines (PAs) and kinetin retarded chlorophyll (Chl) loss, whereas inhibitors of PA biosynthesis [difluoromethyl arginine, difluoromethyl ornithine, methylglyoxal-bis(guanylhydrazone)] and abscisic acid (ABA) promoted senescence. The contents of RNA and protein were significantly higher in PA and kinetin treated leaf discs as compared to those treated with inhibitors and ABA. The contents of total and reducing saccharides declined with the progressive loss of Chl, and the concentration of starch increased in all the PA treated leaf discs. Free amino acid content also increased under all the treatments, but the increase was comparatively larger in case of inhibitors application.

Additional key words

abscisic acid chlorophyll kinetin putrescine RNA spermidine spermine 

Abbreviations

ABA

abscisic acid

CN

control

DFMA

difluoromethyl arginine

DFMO

difluoromethyl omithine

MGBG

methylglyoxal-bis(guanylhydrazone)

Kn

kinetin

PAs

polyamines

Put

putrescine

Spd

spennidine

Spm

spermine

References

  1. Altman, A.: Polyamines and plant hormones. - In: Bachrach, U., Heimer, Y.M. (ed.): The Physiology of Polyamines. Vol.2. Pp. 121–145. CRC Press, Boca Raton 1989.Google Scholar
  2. Apelbaum, A., Goldlust, A., Icekson, I.: Control by ethylene of arginine decarboxylase activity in pea seedlings and its implication for hormonal regulation of plant growth. - Plant Physiol.79: 635–640, 1985.PubMedGoogle Scholar
  3. Barua, D.N.: Science and Practice in Tea Culture. - Tea Research Association, Calcutta-Jorhat 1989.Google Scholar
  4. Cho, S.: Effects of cytokinin and several inorganic cations on the polyamine content of lettuce cotyledons. - Plant Cell Physiol.24: 27–32, 1983.Google Scholar
  5. Cohen, A.S., Popovic, R.B., Zalik, S.: Effects of polyamines on chlorophyll and protein content, photochemical activity, and chloroplast ultrastructure of barley leaf discs during senescence. - Plant Physiol.64: 717–720, 1979.PubMedGoogle Scholar
  6. Drolet, G., Dumbroff, E.B., Legge, R.L., Thompson, J.E.: Radical scavenging properties of polyamines. - Phytochemistry25: 367–371, 1986.CrossRefGoogle Scholar
  7. Dubois, M., Gilles, K.A., Hamilton, J.K., Roberts, P.A., Smith, F.: Colorimetric method for the determination of sugars and related substances. Determination of concentration of pure sugar solution. - Anal. Chem.28: 350–360, 1956.CrossRefGoogle Scholar
  8. Galston, A.W., Altaian, A., Kaur-Sawhney, R.: Polyamines, ribonuclease and the improvement of oat leaf protoplasts. - Plant Sci. Lett.11: 69–79, 1978.CrossRefGoogle Scholar
  9. Galston, A.W., Kaur-Sawhney, R.: Polyamines: are they a new class of plant growth regulators? - In: Wareing, P.F. (ed.): Plant Growth Substances. Pp. 451–461. Academic Press, London 1982.Google Scholar
  10. Galston, A.W., Kaur-Sawhney, R.: Polyamines in plant physiology. - Plant Physiol.94: 406–410, 1990.PubMedGoogle Scholar
  11. Hodge, E.T., Sacher, J.A.: Effects of kinetin, auxin and abscisic acid on ribonuclease and acid phosphatase during senescence of leaf tissue. - Biochem. Physiol. Pflanz.168: 433- 441, 1975.Google Scholar
  12. Isola, M.C., Franzoni, L.: Inhibition of net synthesis of ribonuclease by polyamines in potato tuber slices. - Plant Sci.63: 39–45, 1989.CrossRefGoogle Scholar
  13. Kao, C.H., Yang, S.F.: Role of ethylene in the senescence of detached rice leaves. - Plant Physiol.73: 881–885, 1983.PubMedCrossRefGoogle Scholar
  14. Kaur-Sawhney, R., Galston, A.W.: Physiological and biochemical studies on the anti-senescence properties of polyamines in plants. - In: Slocum, R.D., Flores, H.E. (ed.): Biochemistry and Physiology of Polyamines in Plants. Pp. 201–211. CRC Press, Boca Raton 1991.Google Scholar
  15. Kaur-Sawhney, R., Shih, L.M., Flores, H.E., Galston, A.W.: Relation of polyamine synthesis and titer to aging and senescence in oat leaves. - Plant Physiol.69: 405–410, 1982.PubMedGoogle Scholar
  16. Lalonde, L., Dhindsa, R.S.: Altered protein synthesis duringin situ oat leaf senescence. - Physiol. Plant.80: 619–623, 1990.CrossRefGoogle Scholar
  17. Lee, Y.P., Takahashi, T.: An improved colorimetric determination of amino acids with the use of ninhydrin. - Anal. Biochem.14: 71–72, 1966.CrossRefGoogle Scholar
  18. Lowry, O.H., Rosebrough, N.J., Farr, S.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. - J. biol. Chem.193: 265–275, 1951.PubMedGoogle Scholar
  19. Manivel, L.: Role of maintenance foliage. - Two & A Bud27: 52- 55, 1980.Google Scholar
  20. Markham, R.: Nucleic acid, their components and related compounds. - In: Paech, K., Tracey, M.V. (ed.): Modern Methods of Plant Analysis. Vol.4. Pp. 246–304. Springer-Verlag, Berlin 1955.Google Scholar
  21. McRae, J.C.: Quantitative measurement of starch in very small amounts of leaf tissue. - Planta96: 101–104, 1971.CrossRefGoogle Scholar
  22. Miyazaki, J.H., Yang, S.F.: The methionine salvage pathway in relation to polyamine biosynthesis. - Physiol. Plant.69: 366–370, 1987.CrossRefGoogle Scholar
  23. Naik, B.I., Srivastava, S.K.: Effect of polyamines on tissue permeability. - Phytochemistry17: 1885–1887, 1978.CrossRefGoogle Scholar
  24. Roberts, D.R., Walker, M.A., Thompson, J.E., Dumbroff, E.B.: The effects of inhibitors of polyamine and ethylene biosynthesis on senescence, ethylene production and polyamine levels in cut carnation flowers. - Plant Cell Physiol.25: 315–322, 1984.Google Scholar
  25. Smith, T.A.: Plant polyamines - metabolism and function. - In: Flores, H.E., Arteca, R.N., Shannon, J.C. (ed.): Polyamines and Ethylene: Biochemistry, Physiology and Interactions. Vol.5. Pp. 1–23. American Society of Plant Physiologists, Rockville 1990.Google Scholar
  26. Sumner, J.B.: A more specific reagent for the determination of saccharide in urine. - J. biol. Chem.69: 393, 1935.Google Scholar
  27. Suresh, M.R., Ramakrishna, S., Adiga, P.R.: Regulation of arginine decarboxylase and putrescine levels inCucumis sativus cotyledons. - Phytochemistry17: 57–63, 1978.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, ASCR 1996

Authors and Affiliations

  • R. K. Kakkar
    • 1
  • P. K. Nagar
    • 1
  1. 1.Plant Physiology LaboratoryBiotechnology DivisionPalampurIndia

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