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Biologia Plantarum

, 38:123 | Cite as

Β-Galactosidase in immobilized cells ofPapaver somniferum

  • J. Stano
  • P. Nemec
  • P. Kovács
  • D. Kákoniová
  • D. Liškov’a
  • K. Mičieta
Brief Communication

Abstract

Cell suspension culture of poppy was permeabilized by Tween 80 and immobilized by glutaraldehyde. Β-Galactosidase showed optimum pH 4.8 and temperature of 50 ‡C. The enzyme hydrolysis was linear during 3 h reaching 68 % conversion. The cells characterized by high Β-galactosidase activity and stability in long-term storage showed convenient physico-mechanical properties.

Additional key words

cell immobilization cell suspension glutaraldehyde poppy poppy 

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Copyright information

© Institute of Experimental Botany, ASCR 1996

Authors and Affiliations

  • J. Stano
    • 1
  • P. Nemec
    • 2
  • P. Kovács
    • 2
  • D. Kákoniová
    • 3
  • D. Liškov’a
    • 3
  • K. Mičieta
    • 4
  1. 1.Garden of Medical Plants, Faculty of PharmacyComenius UniversityBratislavaSlovakia
  2. 2.Department of Cell and Molecular Biology of Drugs, Faculty of PharmacyComenius UniversityBratislavaSlovakia
  3. 3.Institute of ChemistrySlovak Academy of SciencesBratislavaSlovakia
  4. 4.Institute of Cell BiologyComenius UniversityBratislavaSlovakia

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