Experimental model ofKlebsiella infection in mice for studies on mechanisms of local immunity of the respiratory tract
- 25 Downloads
An experimental model in white mice, infected with a mildly virulent strain ofKlebsiella pneumoniae, was elaboratored for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intranasal infection. The strain administered by the supralaryngeal route, persisted in the lungs of most mice at approximately equal level 1 d after infection, in some animals it could be demonstrated even after 2–3 d. Using this model (based on various rates of lung clearance), one can demonstrate faster elimination of klebsiella after a local (supralaryngeal) than systemic (intraperitoneal) immunization with a heat-inactivated vaccine, prepared from a homologous strain ofK. pneumoniae.
KeywordsBronchoalveolar Lavage Klebsiella Pneumoniae Squirrel Monkey Lung Clearance Intranasal Infection
Unable to display preview. Download preview PDF.
- Berendt R.F.: Relationship of method of administration to respiratory virulence ofKlebsiella pneumoniae for mice and squirrel monkeys.Infect.Immunity 20, 581 (1978).Google Scholar
- Berendt R.F., Knutsen C.L., Powanda M.C.: Nonhuman primate model for the study of respiratoryKlebsiella pneumoniae infection.Infect.Immunity 22, 275 (1978).Google Scholar
- Berendt R.F., Long G.G., Abeles F.B., Canonico P.G., Elwell M.R., Powanda M.C.: Pathogenesis of respiratoryKlebsiella pneumoniae infection in rats: bacteriological and histological findings and metabolical alterations.Infect.Immunity 15, 586 (1977).Google Scholar
- Franěk J., Fraňková V.:Immunity of Mucous Membrane Surfaces Against Infection. (In Czech) Novinky v medicíně, Vol. 25, Avicenum, Prague 1982.Google Scholar
- Tomasi T.B., Jr.:Immune System of Secretions. Prentice-Hall, Englewood Clifs, New Jersey (USA) 1976.Google Scholar