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Folia Microbiologica

, Volume 28, Issue 5, pp 424–429 | Cite as

Experimental model ofKlebsiella infection in mice for studies on mechanisms of local immunity of the respiratory tract

  • J. Libich
  • J. Franěk
  • V. Hásková
Article

Abstract

An experimental model in white mice, infected with a mildly virulent strain ofKlebsiella pneumoniae, was elaboratored for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intranasal infection. The strain administered by the supralaryngeal route, persisted in the lungs of most mice at approximately equal level 1 d after infection, in some animals it could be demonstrated even after 2–3 d. Using this model (based on various rates of lung clearance), one can demonstrate faster elimination of klebsiella after a local (supralaryngeal) than systemic (intraperitoneal) immunization with a heat-inactivated vaccine, prepared from a homologous strain ofK. pneumoniae.

Keywords

Bronchoalveolar Lavage Klebsiella Pneumoniae Squirrel Monkey Lung Clearance Intranasal Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1983

Authors and Affiliations

  • J. Libich
    • 1
  • J. Franěk
    • 1
  • V. Hásková
    • 1
  1. 1.Institute of Experimental MedicineCzechoslovak Academy of SciencesPrague 2

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