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Golgi 58K-like protein in pollens and pollen tubes ofLilium davidii

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Abstract

In animal cells, Golgi apparatus is located near the microtubule organizing center (MTOC) and its position is determined partly by 58K protein. By sodium dodecyl-sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and immuno-blotting methods, a 58K-like protein has been found in pollen grains and pollen tubes ofLilium davidii. Its molecular weight is very similar to that of the 58K protein of animal cells. By immunofluorescence labeling, under a confocal laser scanning microscope (CLSM), the animal 58K antibody revealed a punctate staining in pollen grains and pollen tubes, which is consistent with the distribution of Golgi apparatus in plant cells. In addition, immuno-gold labeling and transmission electron microscopy showed that the 58K-like protein bound mainly to the membrane of vesicles-like structure near Golgi apparatus. This is the first demonstration of the 58K-like protein in plant cells.

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Authors and Affiliations

  1. College of Biological Sciences, China Agricultural University, China

    Yan Li & Longfei Yan

  2. Key Laboratory of Plant Physiology and Biochemistry, Ministry of Agriculture, 100094, Beijing, China

    Yan Li & Longfei Yan

Authors
  1. Yan Li
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  2. Longfei Yan
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Correspondence to Yan Li.

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Li, Y., Yan, L. Golgi 58K-like protein in pollens and pollen tubes ofLilium davidii . Sci. China Ser. C.-Life Sci. 43, 402–408 (2000). https://doi.org/10.1007/BF02879305

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  • DOI: https://doi.org/10.1007/BF02879305

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