Science in China Series C: Life Sciences

, Volume 43, Issue 4, pp 402–408 | Cite as

Golgi 58K-like protein in pollens and pollen tubes ofLilium davidii



In animal cells, Golgi apparatus is located near the microtubule organizing center (MTOC) and its position is determined partly by 58K protein. By sodium dodecyl-sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and immuno-blotting methods, a 58K-like protein has been found in pollen grains and pollen tubes ofLilium davidii. Its molecular weight is very similar to that of the 58K protein of animal cells. By immunofluorescence labeling, under a confocal laser scanning microscope (CLSM), the animal 58K antibody revealed a punctate staining in pollen grains and pollen tubes, which is consistent with the distribution of Golgi apparatus in plant cells. In addition, immuno-gold labeling and transmission electron microscopy showed that the 58K-like protein bound mainly to the membrane of vesicles-like structure near Golgi apparatus. This is the first demonstration of the 58K-like protein in plant cells.


Golgi apparatus Golgi 58K protein plant pollen 


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Copyright information

© Science in China Press 2000

Authors and Affiliations

  1. 1.College of Biological SciencesChina Agricultural UniversityChina
  2. 2.Key Laboratory of Plant Physiology and BiochemistryMinistry of AgricultureBeijingChina

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