Science in China Series C: Life Sciences

, Volume 40, Issue 1, pp 107–112 | Cite as

Sequence analysis of lacZ- mutations induced by ion beam irradiation in double-stranded M13mp18DNA

  • Jianbo Yang
  • Lijun Wu
  • Li Li
  • Jiadao Wu
  • Zengliang Yu
  • Zhihong Xu


While M13mp18 double-stranded DNA was irradiated with ion beam, and transfected intoE. coli JM103, a decrease of transfecting activity was discovered. The lacZ- mutation frequency at 20% survival could reach (3.6–16.8) × 104, about 2, 3–10 times that of unirradiated M13DNA. Altogether, 27 IacZ-mutants were selected, 10 of which were used for sequencing. 7 of the sequenced mutants show base changes in 250-bp region examined (the remaining 3 mutants probably have base changes outside the regions sequenced). 5 of the base-changed mutants contain more than one mutational base sites (some of them even have 5–6 mutational base sites in 250-bp region examined); this dense distribution of base changes in polysites has seldom been seen in X-rays, Y-rays or UV induced DNA mutations. Our experiments also showed that the types of base changes include transitions(50%), transversions (45%) and deletion (5%); no addition or duplication was observed. The transitions were mainly C→T and A→G; the transversions were mainly C→A and C→G. The mutations involving cytosine residue (in the template strand) constitute about 60% of all the base changes observed. In comparison with the surrounding sequences of mutational base sites, the base located between TG and CT is found to be easily substituted.


ion beam irradiation M13DNA lacZ mutation sequence analysis 


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  1. 1.
    Yu, Z. L., Qiu, L. J., Huo, Y. P., Advances of breeding and biological effect research of ion beam irradiation,J. Anhui Agricultural College (in Chinese), 1991, 18:251.Google Scholar
  2. 2.
    Yu, Z. L., Wang, X. D., Wu, Y. al., Studies for principle of mutation in rice induced by ion beam irradiation,J. Anhui Agricultural Sciences (in Chinese), 1989, 17(1): 12.Google Scholar
  3. 3.
    Wu, Y. J., Yu, Z. L., Dong, J. al., Effect of mutation in rice induced by ion beam irradiation,J. Anhui Agricultural Sciences (in Chinese), 1989, 17(2): 10.Google Scholar
  4. 4.
    Li, L., Yang, J. B., Li, al., Research for structure and functibn of pUC18 DNA irradiated by ion beam,J. Anhui Agricultural Sciences (in Chinese), 1994, 22(4):288.Google Scholar
  5. 5.
    Sambrook, J., Fritsch, E. J., Maniatis, T.,Molecular Cloning—A Laboratory Manual, New York:Cold Spring Harbor Laboratory Press, 1989.Google Scholar
  6. 6.
    Ayaki, H., Higo, K. I., Yamamoto, al., Specificity of ionizing radiation induced mutagenesis in the lac region of single-stranded phage M13mp10 DNA,Nucl. Acids Res., 1986, 14:5013.PubMedCrossRefGoogle Scholar
  7. 7.
    Hoebee, B., Brouwer, J., Putte, P. al.,60Co γ-rays induce predominantly C/G to G/C transversions in double-stranded M13DNA,Nucl. Acids Res., 1988, 16:8147.PubMedCrossRefGoogle Scholar
  8. 8.
    Hoebee, B., Ende, M. A., Brouwer, al., Mutations induced by60Co-γ irradiation in double-stranded M13 bacteriophage DNA in nitrous-oxide saturated solutions are characterized by a high specificity,Int. J. Radiat. Biol., 1989, 56:401.PubMedCrossRefGoogle Scholar
  9. 9.
    Hoebee, B., Loman, K., Brouwer, al., Mutational specificity of γ-rays differs for the same target in plasmid DNA and double-stranded(RF) MI3 bacteriophage DNA,Mutagenesis, 1991, 6:455.PubMedCrossRefGoogle Scholar
  10. 10.
    Bourre, F., Lichtenberger, A., Sarasin, A., Comparison of spontaneous and ultraviolet-induced mutagenesis on naked SV40 DNA and SV40 virus,Mutation Research, 1991, 250:49.PubMedGoogle Scholar
  11. 11.
    Alasdair, J. E., Gordon, E., Moen, C. al., Spontaneous mutagenesis inE. coli harbouring plasmid pKM101 DNA sequence analysis of forward lac I- mutations,Mutagenesis, 1993, 8:133.CrossRefGoogle Scholar
  12. 12.
    Kimura, H., Higuchi, H., Ogawa, H. al., Sequence analysis of X-rays induced mutations Occurring in a cDNA of the human hprt gene integrated into mammalian chromosomal DNA,Radiation Research, 1993, 134:202.PubMedCrossRefGoogle Scholar
  13. 13.
    Yu, Z. L., Shao, C. L., Dose-effect of the tyrosine sample implanted by a low energe N+ ion beam,Radiat. Phys. Chem., 1994, 30:145.Google Scholar

Copyright information

© Science in China Press 1997

Authors and Affiliations

  • Jianbo Yang
    • 1
  • Lijun Wu
    • 1
  • Li Li
    • 1
  • Jiadao Wu
    • 1
  • Zengliang Yu
    • 2
  • Zhihong Xu
    • 3
  1. 1.Institute of RiceAnhui Academy of Agricultural SciencesHefeiChina
  2. 2.Institute of Plasma PhysicsChinese Academy of SciencesHefeiChina
  3. 3.Shanghai Institute of Plant PhysiologyChinese Academy of SciencesShanghaiChina

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