Biologia Plantarum

, Volume 30, Issue 3, pp 179–184 | Cite as

Some factors affecting root formation onin vitro regenerated pea shoots

  • Marie Kubaláková
  • Eva Tejklová
  • M. Griga
Original Paper


The rooting ability of 2 cm long shoots ofPisum sativum L., derived from differentin vitro shoot-tip cultures in two pea cultivars Bohatýr and Kleine Rheinländerin was evaluated. In three mutually independent experiments the full and half-strength Murashige-Skoog medium (containing full or half concentration of macro and microelements), with sucrose concentrations 10–30 g l-1, and with various NAA and IAA combinations, was tested. The variant with half concentration of macro- and microelements, supplemented with 30 g l1 sucrose, and with growth regulators in the quantity of 1 μM proved optimum.


Root Formation Shoot Apex Pisum Sativum Plant Cell Tissue Organ Cult Half Concentration 
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Copyright information

© Academia 1988

Authors and Affiliations

  • Marie Kubaláková
    • 1
  • Eva Tejklová
    • 2
  • M. Griga
    • 2
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesSokolovská 6Czechoslovakia
  2. 2.OSEVA Research and Breeding Institute of Technical Crops and LegumesŠumperk-TemeniceCzechoslovakia

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