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Some factors affecting root formation onin vitro regenerated pea shoots

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Biologia Plantarum

Abstract

The rooting ability of 2 cm long shoots ofPisum sativum L., derived from differentin vitro shoot-tip cultures in two pea cultivars Bohatýr and Kleine Rheinländerin was evaluated. In three mutually independent experiments the full and half-strength Murashige-Skoog medium (containing full or half concentration of macro and microelements), with sucrose concentrations 10–30 g l-1, and with various NAA and IAA combinations, was tested. The variant with half concentration of macro- and microelements, supplemented with 30 g l1 sucrose, and with growth regulators in the quantity of 1 μM proved optimum.

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References

  • Duhoux, E.,Davies, D.: Caulogénèse é partir des bourgeons cotylédonairesd’Acacia albida et influence du saccharose sur la rhizogénèse. - J. Plant Physiol.121: 175–180, 1985.

    CAS  Google Scholar 

  • Gambobg, O. L.,Miller, R. A.,Ojima, K.: Nutrient requirement of suspension cultures of soybean root cells. - Exp. Cell Bes.50: 151–158, 1968.

    Article  Google Scholar 

  • Griga, M.,TbJklová, E.,Novák, F. J.: [Hormonal regulation of growth of pea(Pisum sativum L.) shoot apices inin vitro culture.] In Czech. - Rost. Výroba (Praha)30: 523–630, 1984a.

    CAS  Google Scholar 

  • Griga, M.,Tejklová, E.,Novák, F. J.:In vitro propagation of pea by axillary and adventitious bud technique. - In: Novák, F. J., Havel, L., Doležel, J. (ed.): Plant Tissue and Cell Culture - Application to Crop Improvement. Pp. 507–508. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 1984b.

    Google Scholar 

  • Gbiga, M.,Tejklová, E.,Nováe, F. J.,Kubaláková, M.:In vitro clonal propagation ofPisum sativum L. - Plant Cell Tissue Organ Cult.6: 95–104, 1986.

    Article  Google Scholar 

  • Hunault, G., Leroux, R.: Influence de divers facteurs sur la rhizogenése de tissus d’une mono- cotylédone(Sansevieria trifasciata Prain) et d’une dicotylédone(Pisum sativum L.). - Acteg du 101e Congrès National des Sociétés Savantes, Sciences Fasc. I. Pp. 529–539. Lille 1976.

  • Kartha, K. K.: Culture of shoot meristems: Pea. - In:Vasil, I. K. (ed.): Cell Culture and Somatic Cell Genetics of Plants. Vol. 1. Pp. 106–110, Academic Press, Orlando - San Diego- New York-London-Toronto -Montreal-Sydney-Tokyo 1984.

    Google Scholar 

  • Kartha, K. K.,Gamborg, O. L.,Constabel, F.: Regeneration of pea(Pisum sativum L.) plant3 from shoot apical meristems. - Z. Pflanzenphysiol.72: 172–176, 1974.

    Google Scholar 

  • Kartha, K. K.,Leung, N. L.,Gamborg, O. L.: Freeze-preservation of pea meristems in liquid nitrogen and subsequent plant regeneration. - Plant Sci. Lett.15: 7–15, 1979.

    Article  Google Scholar 

  • Kunakh, V. A.,Alkhimova, E. G.,Voityuk, L. I.: [Variability of the chromosome number in callus tissues and pea regenerants.] In Russ. - Tsitol. Genet.18: 20–26, 1984a.

    Google Scholar 

  • Kunakh, V. A.,Alkhimova, E. G.,Voityuk, L. I,Alpatova, L. K.: Obtaining of tissue cultures and organogenesis induction inPisum sativum L. - In:Novák, F. J.,Havel, L.,Doležel, J. (ed.): Plant Tissue and Cell Culture - Application to Crop Improvement. Pp. 135–136. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 1984b.

    Google Scholar 

  • Kunakh, V. A.,Voityuk, L. I,Alkhimova, E. G.,Alpatova, L. K.: [Callus tissue formation and induction of organogenesis inPisum sativum L.] In Russ. - Fiziol. Rast.31: 452–548, 1984c.

    Google Scholar 

  • Maene, L.,Debergh, P.: Liquid medium additions to established tissue cultures to improve elongation and rootingin vivo. - Plant Cell Tissue Organ Cult.5: 23–33, 1985.

    Article  CAS  Google Scholar 

  • MalMberg, R. L.: Regeneration of whole plants from callus culture of diverse genetic lines ofPisum sativum L. - Planta146: 243–244, 1979.

    Article  Google Scholar 

  • Mroginski, L. A.,Kartha, K. K.: Regeneration of pea(Pisum sativum L. cv. Century) plants byin vitro culture of immature leaflets. - Plant Cell Rep.1: 64–66, 1981.

    Article  Google Scholar 

  • Murashige, T.,Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. -Physiol. Plant.15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nemeth, G.: Benzyladenine-stimulated rooting in fruit-tree rootstock culturedin vitro. - Z. Pflanzenphysiol.95: 389–396, 1979.

    CAS  Google Scholar 

  • Rubluo, A.,Kartha, K. K.,Mroginski, L. A.,Dyck, J.: Plant regeneration from pea leaflets culturedin vitro and genetic stability of regenerants. - J. Plant. Physiol.117: 119–130, 1984.

    CAS  Google Scholar 

  • Rubluo, A.,Mroginski, L. A.,Kartha, K. K.: Morphogenetic responses of pea leaflets culturedin vitro. - In:Fuiwara, A. (ed.): Plant Tissue Culture. Pp. 151–152. Jap. Assoc. Plant Tissue Culture, Tokyo 1982.

    Google Scholar 

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Kubaláková, M., Tejklová, E. & Griga, M. Some factors affecting root formation onin vitro regenerated pea shoots. Biol Plant 30, 179–184 (1988). https://doi.org/10.1007/BF02878753

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  • DOI: https://doi.org/10.1007/BF02878753

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