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Biologia Plantarum

, Volume 30, Issue 3, pp 179–184 | Cite as

Some factors affecting root formation onin vitro regenerated pea shoots

  • Marie Kubaláková
  • Eva Tejklová
  • M. Griga
Original Paper

Abstract

The rooting ability of 2 cm long shoots ofPisum sativum L., derived from differentin vitro shoot-tip cultures in two pea cultivars Bohatýr and Kleine Rheinländerin was evaluated. In three mutually independent experiments the full and half-strength Murashige-Skoog medium (containing full or half concentration of macro and microelements), with sucrose concentrations 10–30 g l-1, and with various NAA and IAA combinations, was tested. The variant with half concentration of macro- and microelements, supplemented with 30 g l1 sucrose, and with growth regulators in the quantity of 1 μM proved optimum.

Keywords

Root Formation Shoot Apex Pisum Sativum Plant Cell Tissue Organ Cult Half Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Academia 1988

Authors and Affiliations

  • Marie Kubaláková
    • 1
  • Eva Tejklová
    • 2
  • M. Griga
    • 2
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesSokolovská 6Czechoslovakia
  2. 2.OSEVA Research and Breeding Institute of Technical Crops and LegumesŠumperk-TemeniceCzechoslovakia

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