Laser-induced bacteriorhodopsin LB films’ fast photoelectric dynamics
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Ten layers of oriented bacteriorhodopsin (BR) LB films are prepared on ITO conductive glass to constitute an ITO electrode/BR LB films/KCl solution/Cu electrode wet photocell. A Q-switched double frequency Nd:YAG laser with 10 Hz repetitive rate and 8 ns pulse width is used to excite the BR LB films. The photoelectric signal is directly captured by a 300 MHz digitizing oscilloscope. A theory on the BR photoelectric dynamics is established. By nonlinear least square fitting of the experimental curves acquired at different time bases, the experimental equation corresponding to the theoretical model is obtained, and the charge displacements and the decay time constants for each step in the BR photocycle are calculated.
Keywordsbacteriorhodopsin LB film photoelectric dynamics
- 2.Haronian, D., Lewis, A., Elements of a unique bacteriorhodopsin neural network architecture,Appl. Opt., 1991, 30(5): 597.Google Scholar
- 4.Simmeth, R., Rayfield, G. W., Evidence that the photoelectric response of bacteriorhodopsin occurs in less than 5 picoseconds,Biophys. J., 1990, 57(5): 1099.Google Scholar