Biologia Plantarum

, Volume 23, Issue 2, pp 91–97 | Cite as

Ontogeny and histochemistry of axillary bud inMurraya koenigii L. spreng

  • T. K. Prasad
  • Y. S. Dave
  • P. M. Mehta
Original Papers


Histochemical localization of total proteins, histories, nucleic acids, ascorbic acid and polysaccharides in the developing axillary bud ofMurraya koenigii and its vascular relationship with the main axis are investigated. All the above variable metabolites are richly distributed throughout the bud development. The shell zone is indicative of poor distribution of these metabolites. Histochemical tests prove that the axillary bud is metabolically very active. The initiation of the axillary bud is in the axil of the 2nd leaf where two cells of 2nd tunica layer become prominent and undergo periclinal divisions to give rise to the bud. The bud maintains tunica-corpus organization throughout its development. The cells of the shell zone at the base of the bud differentiate into pith meristem of the bud. The axillary bud has two bud traces which are associated with vascular strands that are not the traces of the subtending leaf.

Additional index words

Ascorbic acid proteins nucleic acids histones polysaccharides 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Black, M. M., Ansley, H. R.: Histone staining with ammonioal silver. - Science143: 693 to 695, 1964.Google Scholar
  2. Cass, D. D., Jensen, W. A.: Fertilization in barley. - Amer. J. Bot.57: 62–70, 1970.CrossRefGoogle Scholar
  3. Chinoy, J. J.: On the specificity of the alcoholic acidic silver nitrate reagent for the histochemical localization of ascorbic acid. - Histochemie20: 105–107, 1969.PubMedCrossRefGoogle Scholar
  4. Garrison, R.: Studies in the development of axillary buds. - Amer. J. Bot.42: 257–266, 1955.CrossRefGoogle Scholar
  5. Gifford, E. M.: Ontogeny of the vegetative axillary bud inDrimys winteri var.chilensis. - Amer. J. Bot.38: 234–243, 1951.CrossRefGoogle Scholar
  6. Gifford, E. M., Tepper, H. B.: Ontogenetic and histochemical changes in the vegetative shoot tip ofChenopodium album. - Amer. J. Bot.49: 902–911, 1962.CrossRefGoogle Scholar
  7. Jensen, W. A.: Botanical Histochemistry. - Freeman, W. H. and Co., London 1962.Google Scholar
  8. Johansen, D. A.: Plant Microtechnique. - McGraw-Hill, New York 1940.Google Scholar
  9. Majumdar, G. P., Datta, A.: Developmental studies. I. Origin and development of axillary buds with special reference to two dicotyledons. - Proc. indian Acad. Sci.23: 249–259, 1946.Google Scholar
  10. Mazia, D., Brewer, P. A., Alfert, M.: The cytochemical staining and measurement of protein with mercuric bromophenol blue. - Biol. Bull.104: 57–67, 1953.CrossRefGoogle Scholar
  11. Reeve, R. M.: Comparative ontogeny of the inflorescence and the axillary vegetative shoot inGarrya elliptica. - Amer. J. Bot.30: 608–619, 1943.CrossRefGoogle Scholar
  12. Rickson, F. R.: Anatomy, histoohemistry and fine structure of the germinating embryo ofPaulownia tomentosa. - Amer. J. Bot.55: 280–290, 1968.CrossRefGoogle Scholar
  13. Rudramuniappa, C. K., Panchaksharappa, M. D.: Histochemioal study of shoot-root apices inPanicum miliaceum Linn. seedlings. - Proc. indian Acad. Sci.82B: 46–51, 1975.Google Scholar
  14. Rudbamuniappa, C. K., Panchaksharappa, M. D.: Histochemical studies on germinated seeds ofSetaria italica.- Bull. Soc. Bot. Suisse87: 163–172, 1977.Google Scholar
  15. Shah, J. J., Patel, J. D.: Morphohistogenic studies in vegetative and floral buds of brinjal and chilli.- Phytomorphology20: 209–221, 1970.Google Scholar
  16. Shah, J. J., Patel, J. D.: The shell zone: its differentiation and probable function in some dicotyledons.- Amer. J. Bot.59: 683–690, 1972.CrossRefGoogle Scholar
  17. Shah, J. J., Patel, J. D., Kothari, I. L.: Vascular supply of axillary buds in some dicotyledons.- Adv. Plant Morphol. Pp. 65–75. Sarita prakashan, Meerut, India 1972.Google Scholar
  18. Shah, J. J., Unnikrishnan, K.: Ontogeny of axillary and accessory buds inClerodendrum phlomides L. - Ann. Bot.33: 389–398, 1969.Google Scholar
  19. Tucker, S. C.: Development and phyllotaxis of the vegetative axillary bud ofMichelio fuscato. - Amer. J. Bot.50: 661–668, 1963.CrossRefGoogle Scholar
  20. Wang, P. J.: Histochemical studies of the shoot apex inBrassica chinensis cv. Tope-Tsai. - Bot. Bull. Aoad. sin.12: 31–40, 1971.Google Scholar

Copyright information

© Academia 1981

Authors and Affiliations

  • T. K. Prasad
    • 1
  • Y. S. Dave
    • 1
  • P. M. Mehta
    • 1
  1. 1.Department of BiosciencesSardar Patel UniversityVallabh VidyanagarIndia

Personalised recommendations