Abstract
Histochemical localization of total proteins, histories, nucleic acids, ascorbic acid and polysaccharides in the developing axillary bud ofMurraya koenigii and its vascular relationship with the main axis are investigated. All the above variable metabolites are richly distributed throughout the bud development. The shell zone is indicative of poor distribution of these metabolites. Histochemical tests prove that the axillary bud is metabolically very active. The initiation of the axillary bud is in the axil of the 2nd leaf where two cells of 2nd tunica layer become prominent and undergo periclinal divisions to give rise to the bud. The bud maintains tunica-corpus organization throughout its development. The cells of the shell zone at the base of the bud differentiate into pith meristem of the bud. The axillary bud has two bud traces which are associated with vascular strands that are not the traces of the subtending leaf.
Similar content being viewed by others
References
Black, M. M., Ansley, H. R.: Histone staining with ammonioal silver. - Science143: 693 to 695, 1964.
Cass, D. D., Jensen, W. A.: Fertilization in barley. - Amer. J. Bot.57: 62–70, 1970.
Chinoy, J. J.: On the specificity of the alcoholic acidic silver nitrate reagent for the histochemical localization of ascorbic acid. - Histochemie20: 105–107, 1969.
Garrison, R.: Studies in the development of axillary buds. - Amer. J. Bot.42: 257–266, 1955.
Gifford, E. M.: Ontogeny of the vegetative axillary bud inDrimys winteri var.chilensis. - Amer. J. Bot.38: 234–243, 1951.
Gifford, E. M., Tepper, H. B.: Ontogenetic and histochemical changes in the vegetative shoot tip ofChenopodium album. - Amer. J. Bot.49: 902–911, 1962.
Jensen, W. A.: Botanical Histochemistry. - Freeman, W. H. and Co., London 1962.
Johansen, D. A.: Plant Microtechnique. - McGraw-Hill, New York 1940.
Majumdar, G. P., Datta, A.: Developmental studies. I. Origin and development of axillary buds with special reference to two dicotyledons. - Proc. indian Acad. Sci.23: 249–259, 1946.
Mazia, D., Brewer, P. A., Alfert, M.: The cytochemical staining and measurement of protein with mercuric bromophenol blue. - Biol. Bull.104: 57–67, 1953.
Reeve, R. M.: Comparative ontogeny of the inflorescence and the axillary vegetative shoot inGarrya elliptica. - Amer. J. Bot.30: 608–619, 1943.
Rickson, F. R.: Anatomy, histoohemistry and fine structure of the germinating embryo ofPaulownia tomentosa. - Amer. J. Bot.55: 280–290, 1968.
Rudramuniappa, C. K., Panchaksharappa, M. D.: Histochemioal study of shoot-root apices inPanicum miliaceum Linn. seedlings. - Proc. indian Acad. Sci.82B: 46–51, 1975.
Rudbamuniappa, C. K., Panchaksharappa, M. D.: Histochemical studies on germinated seeds ofSetaria italica.- Bull. Soc. Bot. Suisse87: 163–172, 1977.
Shah, J. J., Patel, J. D.: Morphohistogenic studies in vegetative and floral buds of brinjal and chilli.- Phytomorphology20: 209–221, 1970.
Shah, J. J., Patel, J. D.: The shell zone: its differentiation and probable function in some dicotyledons.- Amer. J. Bot.59: 683–690, 1972.
Shah, J. J., Patel, J. D., Kothari, I. L.: Vascular supply of axillary buds in some dicotyledons.- Adv. Plant Morphol. Pp. 65–75. Sarita prakashan, Meerut, India 1972.
Shah, J. J., Unnikrishnan, K.: Ontogeny of axillary and accessory buds inClerodendrum phlomides L. - Ann. Bot.33: 389–398, 1969.
Tucker, S. C.: Development and phyllotaxis of the vegetative axillary bud ofMichelio fuscato. - Amer. J. Bot.50: 661–668, 1963.
Wang, P. J.: Histochemical studies of the shoot apex inBrassica chinensis cv. Tope-Tsai. - Bot. Bull. Aoad. sin.12: 31–40, 1971.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Prasad, T.K., Dave, Y.S. & Mehta, P.M. Ontogeny and histochemistry of axillary bud inMurraya koenigii L. spreng. Biol Plant 23, 91–97 (1981). https://doi.org/10.1007/BF02878410
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02878410