Abstract
During 6 days of pea seed germination the depletion of legumin with mol. m. 390 000 from protein bodies was observed. SDS-PAGE indicated that the legumin subunits with mol. m. 41 700 and 21 000 were prevailing. Only the former of these, probably corresponding to α-subunit, was degraded rapidly during 6 days of germination.
Water-soluble proteins (albumins) prepared from pea cotyledons were separated by preparative IEF into proteins with pI 7.1, 6.5, 6.0, 5.4, 5.0, and 4.6. During 6 days the components of albumin with pI 7.1, and 6.5 were dramatically depleted. Major fractions with pI 6.5, 6.0, and 5.4 were subjected to SDS-PAGE and their subunit composition was determined. Moreover, albumin of pea cotyledons was resolved into 13 components by SDS-PAGE. Mobilization of albumin began from the degradation of components with higher mol. m. during germination.
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Abbreviations
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel eleetrophoresis
- IEF:
-
isoelectric focusing
- TCA:
-
trichloroacetic acid
References
Basha, S. M. M., Beevers, L.: The development of proteolytic activity and protein degradation during the germination ofPisum sativum L. - Planta124: 77–87, 1975.
Casey, R.: Immunoaffinity chromatography as a means of purifying legumin fromPisum (pea) seeds. - Bioehem. J.177: 509–520, 1979.
Croy, R. R. D., Derbyshire, E., Krishna, T. G., Boulter, D.: Legumin ofPisum sativum andVicia faba. - New Phytol.83: 29–35, 1979.
Croy, R. R. D., Gatehouse, J. A., Evans, I. M., Boulter, D.: Characterization of the storage protein subunits synthesizedin vitro by polyribosomes and RNA from developing pea (Pisum sativum L). I. Legumin. - Planta148: 49–56, 1980.
Gatehouse, J. A., Croy, R. R. D., Boulter, D.: Isoelectric focusing properties and carbohydrate content of pea (Pisum sativum) legumin. - Biochem. J.185: 497–503, 1980.
Johnston, A. W. B., Brewster, V., Davies, D. R.: Seed proteins of peas in relation to nitrogen fixation. - Ann. Bot.41: 381–385, 1977.
Konopska, L.: Nitrogen and phosphorus compounds in the aleurone grains ofIris pseudoacorus endosperm andPisum sativum cotyledons. - Acta Soc. Bot. Pol.42: 533–540, 1973.
Konopska, L.: Proteins of aleurone grains in germinating seeds ofPisum sativum L. - Biochem. Physiol. Pflanzen173: 322–326, 1978.
Konopska, L.: Changes in protein composition of aleurone grains ofPisum sativum L. during germination. - Bioehem. Physiol. Pflanzen174: 275–282, 1979.
Liohtenfeld, C., Manteuffel, R., Müntz, K.:In vivo andin vitro proteolysis of storage proteins by proteases from germinatingVicia faba seeds. - 2nd Symposium on Seed Proteins, Gatersleben, Poster no. 28. 1980.
Osborne, T. B.: The vegetable proteins. - Longman Gr. Comp., London 1924.
Righetti, P. G., Drysdale, J. W.: Preparative IEF. - In:Work, T. S., Work, E. (ed.): Isoelectric focusing. Pp. 377–418. North-Holland Publishing Company, Amsterdam-Oxford-New York 1976.
Thomson, J. A., Schroeder, H. E., Dudman, W. F.: Cotyledonary storage proteins inPisum sativum. I. Molecular heterogeneity. - Aust. J. Plant Physiol.5: 263–279, 1978.
Weber, K., Osborn, M.: The reliability of molecular weight determinations by dodecyl sulfatepolyacrylamide gel electrophoresis. - J. biol. Chem.244: 4406–4412, 1969.
Weber, K., Pringle, J. R., Osborn, M.: Measurement of molecular weights by electrophoresis on SDS acrylamide gel. - Methods Enzymol.26: 3–27, 1972.
Youle, R. J., Huang, A. H. C.: Albumin storage proteins in the protein bodies of castor bean. - Plant Physiol.61: 13–16, 1978.
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Konopska, L. Legumin and albumin of pea cotyledons during seed germination. Biol Plant 25, 15–20 (1983). https://doi.org/10.1007/BF02878261
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DOI: https://doi.org/10.1007/BF02878261