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Biologia Plantarum

, Volume 30, Issue 4, pp 247–251 | Cite as

Karyotype stability in long-term callus derived plants ofCrepis tectorum L.

  • J. Sengupta
  • S. Jha
  • S. Sen
Original paper

Abstract

The study of in vitro growth of Crepis tectorum revealed 100 % callusing and 40 % plantlet regeneration. The root and leaf used as explants showed the normal diploid (2n=8) chromosome constitution. In one month old culture 95 % callus cells were diploid. The callus maintained in 2,4-D 1 mg 1-1 for two years showed 62 % diploid, 5 % tetraploid and 33 % hyperdiploid cells. The differentiation of shoot occurred in two year old calli after subeulturing in 2 mg I-1 BAP and the potentiality of regeneration was retained for more than one year. The leaf-tips of regenerated plants were homogeneous and identical to the donor plant both in number and morphology of chromosomes.

Keywords

Diploid Cell Donor Plant Plantlet Regeneration Chromosomal Constitution Polyploid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Academia 1988

Authors and Affiliations

  • J. Sengupta
    • 1
  • S. Jha
    • 1
  • S. Sen
    • 1
  1. 1.Centre of Advanced Study, Department of BotanyUniversity of CalcuttaCalcuttaIndia

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