Biologia Plantarum

, Volume 30, Issue 4, pp 247–251 | Cite as

Karyotype stability in long-term callus derived plants ofCrepis tectorum L.

  • J. Sengupta
  • S. Jha
  • S. Sen
Original paper


The study of in vitro growth of Crepis tectorum revealed 100 % callusing and 40 % plantlet regeneration. The root and leaf used as explants showed the normal diploid (2n=8) chromosome constitution. In one month old culture 95 % callus cells were diploid. The callus maintained in 2,4-D 1 mg 1-1 for two years showed 62 % diploid, 5 % tetraploid and 33 % hyperdiploid cells. The differentiation of shoot occurred in two year old calli after subeulturing in 2 mg I-1 BAP and the potentiality of regeneration was retained for more than one year. The leaf-tips of regenerated plants were homogeneous and identical to the donor plant both in number and morphology of chromosomes.


Diploid Cell Donor Plant Plantlet Regeneration Chromosomal Constitution Polyploid Cell 
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Copyright information

© Academia 1988

Authors and Affiliations

  • J. Sengupta
    • 1
  • S. Jha
    • 1
  • S. Sen
    • 1
  1. 1.Centre of Advanced Study, Department of BotanyUniversity of CalcuttaCalcuttaIndia

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