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Karyotype stability in long-term callus derived plants ofCrepis tectorum L.

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Biologia Plantarum

Abstract

The study of in vitro growth of Crepis tectorum revealed 100 % callusing and 40 % plantlet regeneration. The root and leaf used as explants showed the normal diploid (2n=8) chromosome constitution. In one month old culture 95 % callus cells were diploid. The callus maintained in 2,4-D 1 mg 1-1 for two years showed 62 % diploid, 5 % tetraploid and 33 % hyperdiploid cells. The differentiation of shoot occurred in two year old calli after subeulturing in 2 mg I-1 BAP and the potentiality of regeneration was retained for more than one year. The leaf-tips of regenerated plants were homogeneous and identical to the donor plant both in number and morphology of chromosomes.

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Authors and Affiliations

  1. Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, 700 019, Calcutta, India

    J. Sengupta, S. Jha & S. Sen

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  1. J. Sengupta
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  2. S. Jha
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  3. S. Sen
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Sengupta, J., Jha, S. & Sen, S. Karyotype stability in long-term callus derived plants ofCrepis tectorum L.. Biol Plant 30, 247–251 (1988). https://doi.org/10.1007/BF02878202

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  • DOI: https://doi.org/10.1007/BF02878202

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