Overcoming interspecific incompatibility in the crossBrassica campestris ssp.japonica xBrassica oleracea var.botrytis using irradiated mentor pollen
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The cross B. campestris ssp. japonica x B. oleracea var. botrytis fails due to incompatibility barrier at the stigma. To realize this cross, irradiated compatible pollen (mentor pollen) was used before the incompatible pollination. The presence of mentor pollen stimulated the incompatible pollen to germinate and effect fertilization and seed set. One hybrid was thus obtained. Most of the seeds were inviable. Of the 5 plants raised one was a hybrid and 4 resembled the female parent.
KeywordsPollen Tube Female Parent Pollen Germination Aniline Blue Indian Agricultural Research Institute
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