Advertisement

Biologia Plantarum

, Volume 22, Issue 1, pp 1–6 | Cite as

Effect of maleic hydrazide on peroxidase isoenzymes in relation to rooting hypocotyl cuttings ofPhaseolus mungo

  • R. N. Chibbar
  • K. Gurumurti
  • K. K. Nanda
Original Papers
  • 27 Downloads

Abstract

A time course study of changes in the pattern of peroxidase isoenzymes shows that two new isoenzymesa andb appeared in hypocotyl cuttings cultured either in indolyl-3 acetic acid (IAA) + sucrose or in water but not in maleic hydrazide (MH). Roots were also initiated in the former two cases but not in the last case. That these isoenzymes may be associated with root initiation is also evident from the fact that these appeared when hypocotyl cuttings were transferred from MH to IAA + sucrose. The time of appearance of these isoenzymes in different cultures also synchronized with the time of microscopic root initiation. While the isoenzymea disappeared,b persisted in cuttings transferred from IAA + sucrose to MH. The number of roots produced on cuttings transferred to MH was very low, though their development was normal. It is suggested that isoenzymesa andb may be associated with root initiation andc andd with root development.

Keywords

Adventitious Root Root Initiation Forest Research Institute Maleic Hydrazide Maleic Hydrazide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chandra, G. R., Gregory, L. E., Worley, J. F.: Studies on the initiation of adventitious roots on mung bean hypocotyl. — Plant Cell Physiol.12: 317–324, 1971.Google Scholar
  2. Endo, T.: Indoleacetate oxidase activity of horseradish and other plant peroxidase isoenzymes. — Plant Cell Physiol.9: 333–341, 1968.Google Scholar
  3. Gurumurti, K., Nanda, K. K.: Changes in peroxidase isoenzymes ofPhaseolus mungo hypocotyl cuttings during rooting. — Phytochemistry13: 1089–1093, 1974.CrossRefGoogle Scholar
  4. Kihlmann, B. A.: Action of Chemicals on Dividing Cells. — Prentice Hall Inc., New Jersey 1966.Google Scholar
  5. Nanda, K. K., Bhattacharya, N. C., Kaur, N. P.: Effect of morphactin on peroxidases and its relationship to rooting hypocotyl cuttings ofImpatiens balsamina. — Plant Cell Physiol.14: 207–211, 1973.Google Scholar
  6. Nanda, K. K., Bhattacharya, N. C., Kochhar, V. K.: Biochemical basis of adventitious root formation on stem cuttings. — N.Z.J. Forest Sci.4: 347–358, 1974.Google Scholar
  7. Nooden, L. D.: The mode of action of maleic hydrazide: Inhibition of growth. — Physiol. Plant.22: 260–270, 1969.CrossRefGoogle Scholar
  8. Webb, J. L.: Enzyme and Metabolic Inhibitors, Vol. III. Pp. 326–335. — Academic Press, New York-London 1966.Google Scholar
  9. Yoneda, Y., Endo, T.: Peroxidase activity and their indoleacetate oxidase activity in the Japanese morning glory,Pharbitis nil. — Plant Cell Physiol.11: 503–506, 1970.Google Scholar

Copyright information

© Academia 1980

Authors and Affiliations

  • R. N. Chibbar
    • 1
  • K. Gurumurti
    • 1
  • K. K. Nanda
    • 1
  1. 1.Department of BotanyPanjab UniversityChandigarhIndia

Personalised recommendations