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Science in China Series B: Chemistry

, Volume 41, Issue 2, pp 135–141 | Cite as

Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane

  • Kunsheng Hu
  • Jin’an He
  • Hengtao Zhang
Article

Abstract

The steady absorption and kinetic changes of M412 intermediate of the light- and dark-adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X-100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X-100. The destruction to the cooperative effect of BR ultimately caused 13-cis-retinal of the dark-adapted BR to take part in BR photocycle and also to generate the deprotonated M412 intermediate.

Keywords

bacteriorhodopsin purple membrane cooperative effect Triton X-100 M412 intermediate 

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References

  1. 1.
    Simon, M. I., Strathmann, M. P., Gautam, N., Diversity of G protein in signal transduction,Science, 1991, 252: 802.CrossRefGoogle Scholar
  2. 2.
    Lanyi, J. K., Proton translocation mechanism and energetics in the light-driven pump bacteriorhodopsin,Biuchim. Biophys. Acta, 1993, 1183: 241.CrossRefGoogle Scholar
  3. 3.
    El-Sayed, M. A., On the molecular mechanisms of the solar to electric energy conversion by the other photosynthetic system in nature, bacteriorhodopsin,Acc. Chem. Res., 1992, 25: 279.CrossRefGoogle Scholar
  4. 4.
    Tokaji, Z., Cooperativity-regulated parallel pathways of the bacteriorhodopsin photocycle,FEBS Letters, 1995, 357: 156.CrossRefGoogle Scholar
  5. 5.
    Rothschild, K. J., FTIR difference spectroscopy of bacteriorhodopsin: toward a molecular model,J. Bioenerg. Biomembr., 1992, 24: 147.CrossRefGoogle Scholar
  6. 6.
    Shrager, R. I., Hendler, R. W., Bose, S., The ability of actinic light to modify the bacteriorhodopsin photocycle hetemgeneity and/or photocooperative effect?Eur. J. Biochem., 1995, 229: 589.CrossRefGoogle Scholar
  7. 7.
    Braiman, M. S., Mathies, R., Resonance Raman spectroscopy of bacteriorhodopsin’s primary photoproduct: evidence for a distorted 13-cis retinal chromophore,Proc. Natl. Acad. Sci. USA, 1982, 79: 403.CrossRefGoogle Scholar
  8. 8.
    Tsuda, M., Glaccum, M., Nelson B. et al., Light isomerizes the chromophore of bacteriorhodopsin,Nature, 1980, 287: 351.CrossRefGoogle Scholar
  9. 9.
    Kaulen, A. D., Drachev, L. A., Zorina, V. V., Proton transport and M-type intermediate formation by 13-cis-bacteriorhodopsin,Biochim. Biophys. Acta, 1990, 1018: 103.CrossRefGoogle Scholar
  10. 10.
    Oesterhelt, D., Stoeckenius, W., Isolation of the cell membrane ofHalobacterium halobium and its fraction into red and blue membranes,Methods Enzymol., 1974, 31A: 667.CrossRefGoogle Scholar
  11. 11.
    Govindjee, R., Ebrey, T. G., Crofts, A. R., The quantum efficiency of proton pumping by the purple membrane ofHalohacterium halobium, Biophys. J., 1980, 30: 231.CrossRefGoogle Scholar
  12. 12.
    Jiang, Q. X., Hu, K. S., Wang, W., pH-dependence of interaction between melittin and bacteriorhodopsin.Science in China, Ser.C, 1996, 39: 362.Google Scholar
  13. 13.
    Viguera, A. R., Gonzalez-Manas, J. M., Taneva, S. et al., Early and delayed stages in the solubilization of purple membrane by a polyoxyethylenic surfactant,Biochim. Biophys. Acta, 1994, 1196: 76.CrossRefGoogle Scholar
  14. 14.
    Mukhopadhyay, A. K., Dracheva, S., Bose, S. et al., Control of the integral membrane proton pump, bacteriorhodopsin, by purple membrane lipids ofHalobacterium halobium, Biochemistry, 1996, 35: 9245.CrossRefGoogle Scholar
  15. 15.
    Dracheva, S., Bose, S., Hendler, R. W., Chemical and functional studies on the importance of purple membrane lipids in bacteriorhodopsin photocycle behavior,FEBS Letters, 1996, 382:209.CrossRefGoogle Scholar

Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Kunsheng Hu
    • 1
  • Jin’an He
    • 1
  • Hengtao Zhang
    • 1
  1. 1.Department of Cell Biology, Institute of BiophysicsChinese Academy of SciencesBeijingChina

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