Science in China Series B: Chemistry

, Volume 41, Issue 2, pp 135–141 | Cite as

Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane

  • Kunsheng Hu
  • Jin’an He
  • Hengtao Zhang


The steady absorption and kinetic changes of M412 intermediate of the light- and dark-adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X-100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X-100. The destruction to the cooperative effect of BR ultimately caused 13-cis-retinal of the dark-adapted BR to take part in BR photocycle and also to generate the deprotonated M412 intermediate.


bacteriorhodopsin purple membrane cooperative effect Triton X-100 M412 intermediate 


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Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Kunsheng Hu
    • 1
  • Jin’an He
    • 1
  • Hengtao Zhang
    • 1
  1. 1.Department of Cell Biology, Institute of BiophysicsChinese Academy of SciencesBeijingChina

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