Folia Microbiologica

, Volume 28, Issue 2, pp 100–105 | Cite as

Production of lyases byPhoma exigua associated with seed-rot ofVigna radiata

  • M. A. S. Charya
  • S. M. Reddy


Phoma exigua associated with seed-rot ofVigna radiata produced lyases which varied with the media tested. The production of lyases was higher in pectin-supplemented media.Vigna seed meal medium was not suitable for induction of lyase production. The pectin lyase and pectate lyase was maximum after 11 d of incubation by which time the pH was shifted to alkaline side. Temperature of 25 °C and pH 9 was found to be optimum for the activity of pectin lyase and pectate lyase. Fungicides (antracol and panoctine), phenols (pyrocatechol and gallic acid) and growth substances (gibberellic acid and yeast extract) adversely affected the enzyme secretion.


Pectin Gallic Acid Pyrocatechol Pectin Lyase Pectic Enzyme 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1983

Authors and Affiliations

  • M. A. S. Charya
    • 1
  • S. M. Reddy
    • 1
  1. 1.Department of BotanyKakatiya UniversityWarangalIndia

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