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Production of lyases byPhoma exigua associated with seed-rot ofVigna radiata

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Abstract

Phoma exigua associated with seed-rot ofVigna radiata produced lyases which varied with the media tested. The production of lyases was higher in pectin-supplemented media.Vigna seed meal medium was not suitable for induction of lyase production. The pectin lyase and pectate lyase was maximum after 11 d of incubation by which time the pH was shifted to alkaline side. Temperature of 25 °C and pH 9 was found to be optimum for the activity of pectin lyase and pectate lyase. Fungicides (antracol and panoctine), phenols (pyrocatechol and gallic acid) and growth substances (gibberellic acid and yeast extract) adversely affected the enzyme secretion.

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Authors and Affiliations

  1. Department of Botany, Kakatiya University, 506 009, Warangal, India

    M. A. S. Charya & S. M. Reddy

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  1. M. A. S. Charya
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  2. S. M. Reddy
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Charya, M.A.S., Reddy, S.M. Production of lyases byPhoma exigua associated with seed-rot ofVigna radiata . Folia Microbiol 28, 100–105 (1983). https://doi.org/10.1007/BF02877364

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  • DOI: https://doi.org/10.1007/BF02877364

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