Advertisement

Folia Microbiologica

, Volume 28, Issue 2, pp 100–105 | Cite as

Production of lyases byPhoma exigua associated with seed-rot ofVigna radiata

  • M. A. S. Charya
  • S. M. Reddy
Article

Abstract

Phoma exigua associated with seed-rot ofVigna radiata produced lyases which varied with the media tested. The production of lyases was higher in pectin-supplemented media.Vigna seed meal medium was not suitable for induction of lyase production. The pectin lyase and pectate lyase was maximum after 11 d of incubation by which time the pH was shifted to alkaline side. Temperature of 25 °C and pH 9 was found to be optimum for the activity of pectin lyase and pectate lyase. Fungicides (antracol and panoctine), phenols (pyrocatechol and gallic acid) and growth substances (gibberellic acid and yeast extract) adversely affected the enzyme secretion.

Keywords

Pectin Gallic Acid Pyrocatechol Pectin Lyase Pectic Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Agarwal G.P., Pushpa: Comparison of the effect of different metalic and non-metalic salts on the pectolytic enzymes produced by three isolates ofColletotrichum on fruits during pathogenesis. All India Symp.Physiology of Parasitism, University of Jabalpur 1978.Google Scholar
  2. Albershfim P., Killas V.: Studies relating to the purification and properties on pectin transeliminase.Arch. Biochem. Biophys.97, 107 (1962).CrossRefGoogle Scholar
  3. Asthana R.P., Hawker L.E.: The influence of certain fungi on the sporulation ofMelanospora destruens and some other ascomyeetes.Ann. Bot. London50, 325 (1936).Google Scholar
  4. Bateman D.F., Millfr R.L.: Pectic enzymes in tissue degradation.Ann. Rev. Phytopath.4, 119 (1966).CrossRefGoogle Scholar
  5. Bordia, Snehtlata, Dube M.C.: Extracellular pectolytic enzymes ofHelminthosporium sacchari, the incitant of Eye-spot disease of sugar cane. All India Symp.Physiology of Parasitism, University of Jabalpur 1978.Google Scholar
  6. Bughbee W.M.: Pectolytic enzymes production byPhoma betae.Can. J.Bot.50, 1705 (1972).CrossRefGoogle Scholar
  7. Charya M.A.S., Reddy S.M.: Production of cell wall degrading enzymes by two seed-borne fungi.Curr. Sci.49, 557 (1980).Google Scholar
  8. Dube H.C., Mathur S.: Hydrolytic and trans-eliminative degradation of pectic substances by extracellular enzymes ofVerticillium dahliae.Indian Phytopath.28, 346 (1975).Google Scholar
  9. Edstrom R.D., Phaff H.J.: Purification and certain properties of pectic trans-eliminase fromAspergillus fonsecaeus.J. Biol. Chem.239, 2403 (1964).PubMedGoogle Scholar
  10. Garrett S.D.: Soil conditions and the take-all disease of wheat.Ann. Appl. Biol.23, 667 (1936).CrossRefGoogle Scholar
  11. Grovep R.K., Moore M.D.: Taximetric studies of fungicides against brown rot organismSclerotinia fructicola andS. laxa.Phytopathol.52, 876 (1962).Google Scholar
  12. Gupta S.C., Prasad V.: Studies in pectic enzymes of parasitic fungi. XI. Inhibitory effect of some fungicides on production and activity of pectic enzymes ofAlternaria tenuis.Agra Univ. J. Res.17, 161 (1969).Google Scholar
  13. Hancock J.G., Miller R.L.: Relative importance of galacturonate trans-eliminase and other pectolytic enzymes in southern anthracnose. Spring black stem andStempylium leaf-rot of alfalfa.Phytopathol.55, 346 (1965).Google Scholar
  14. Hsu E.J., Vaughan R.H.: Production and catabolite repression of the constitutive polygalacturonic acid trans-eliminase ofAeromonas liguefaciens.J. Bacterial.98, 172 (1969).Google Scholar
  15. Humter W.J., Elkan G.H.: Role of pectic and cellulolytic enzymes in the invasion of the soybean byRhizobium japonicum.Can. J. Microbiol.21, 1254 (1975).CrossRefGoogle Scholar
  16. Olujtiola P.O., Akintude O.A.: Pectic lyase and pectin methylesterase production byPenicillium citrinum.Trans. Brit. Mycol. Soc.72, 49 (1979).Google Scholar
  17. Reddy S.M., Laxminarayana P.: Production of extracellular pectic enzymes by three fruit-rot fungi. All India Symp.Physiology of Parasitism, University of Jabalpur 1978.Google Scholar
  18. Sherwood R.T.: Pectin lyase and polygalacturonase production byRhizoctonia solani and other fungi.Phytopathol.56, 279 (1967).Google Scholar
  19. Talboys P.W., Busch L.V.: Pectic enzymes produced byVerticillium species.Trans. Brit. Mycol. Soc.55, 367 (1970).CrossRefGoogle Scholar
  20. Thirupathaiah V., Subramanian D.: Sporulation and polygalacturonase trans-eliminase production byColletotrichum capsici (Syd.) Butl. & Bisby.Phytopathol.75, 175 (1972).Google Scholar
  21. Vidhyasekaran P.: Possible mode of action of phenolics in inducing disease resistance in Ragi plants againstHelminthosporium tetramera.Indian Phytopath.27, 587 (1974).Google Scholar
  22. Wood R.K.S.: Pectic enzymes secreted by pathogens and their role in plant infection, p. 263 inMechanism of Microbial Pathogenesity (J.W. Howie, A.J.O. Hea, Eds). University Press, Cambridge 1955.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1983

Authors and Affiliations

  • M. A. S. Charya
    • 1
  • S. M. Reddy
    • 1
  1. 1.Department of BotanyKakatiya UniversityWarangalIndia

Personalised recommendations