Formation of poly-3-hydroxybutyrate by a soil microbial community during batch and heterocontinuous cultivation
Glucose added to soil as an extracellular source of carbon and energy was proved to be deposited into an intracellular polymer poly-3-hydroxybutyrate (PHB). Untreated soil samples contained PHB amounts corresponding to 1.56 –2.64 μg of crotonic acid per 1 g soil. During batch cultivation after addition of 1 % glucose the PHB content increased by 20-fold after 2 d and then decreased owing to the disappearance of glucose from the soil. Repeated additions of glucose did not bring about any significant increase in PHB content as compared with a single addition. In soil supplied continuously with 0.1 or 0.25 % glucose solution, the content of PHB increased, after an initial lag, gradually up to the 10th day. After 1-d cultivation the content of PHB in the batch system increased even in the presence of diammonium hydrogen phosphate. In a heterocontinuous system no PHB accumulation took place in the presence of this source of nitrogen and phosphorus as long as the C:N ratio of theadded substrate was 10: 1.
KeywordsBatch System Repeated Addition Crotonic Acid Coryneform Bacterium Hydroxybutyric Acid
Unable to display preview. Download preview PDF.
- Boylen C.W., Mulks M.H.: Coryneform bacteria during periods of prolonged nutrient starvation.J. Gen.Microbiol.105, 323 (1978).Google Scholar
- Doudoroff M.: Metabolism of poly-β-hydroxybutyrate in bacteria, p. 385 inCurrent Aspects of Biochemical Energetics (N.O. Kaplan, E.P. Kennedy, eds.). Academic Press, New York-London 1966.Google Scholar
- Jarnan T.R., Deavin L., Scolombe S., Righelato R.C.: Investigation of the effect of environmental conditions on the rate of exopolysaccharide synthesis inAzotobacter vinelandii.J.Gen. Microbiol.107, 59 (1978).Google Scholar
- Kunc F.: Study of conversions of some organic substrates in soil. (In Czech)PhD Thesis, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague1965.Google Scholar
- Macura J.: Heterocontinuous cultivation of microorganisms in soil. (In Czech)DSc Thesis, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 1969.Google Scholar
- Nuti M.P., De Bertoldi M., Lepidi A.A.: A simple method of extraction of poly-β-hydroxybutyrate from aerobic and anaerobic soil bacteria.Biologie du Sol15, 6 (1972).Google Scholar
- Slepecky R.A., Law J.H.: Synthesis and degradation of poly-β-hydroxybutyric acid in connection with sporulationof Bacillus megaterium.J. Bacterial.82, 37 (1961).Google Scholar
- Veldkamp H.: Bacterial physiology. p. 201 inThe Ecology of Soil Bacteria (T.R.G. Gray, D. Parkinson, eds.). Liverpool University Press, Liverpool 1968.Google Scholar
- Waschko M.E., Rice E.W.: Determination of glucose by an improved “Glucostat” procedure.Clin.Chem.7, 542 (1961).Google Scholar
- Wilkinson J.F., Munro A.L.S.: The influence of growth limiting conditions on the synthesis of possible carbon and energy storage polymers inBacillus megaterium, p. 173 inMicrobial Physiology and Continuous Culture (E.O. Powell, C.G.T. Evans, R.E. Strange, D.W. Tempest, eds.). Her Majesty’s Stationary Office, London 1967.Google Scholar