Folia Microbiologica

, Volume 29, Issue 3, pp 233–241 | Cite as

Formation of poly-3-hydroxybutyrate by a soil microbial community during batch and heterocontinuous cultivation

  • A. Hanzlíkova
  • A. Jandera
  • F. Kunc


Glucose added to soil as an extracellular source of carbon and energy was proved to be deposited into an intracellular polymer poly-3-hydroxybutyrate (PHB). Untreated soil samples contained PHB amounts corresponding to 1.56 –2.64 μg of crotonic acid per 1 g soil. During batch cultivation after addition of 1 % glucose the PHB content increased by 20-fold after 2 d and then decreased owing to the disappearance of glucose from the soil. Repeated additions of glucose did not bring about any significant increase in PHB content as compared with a single addition. In soil supplied continuously with 0.1 or 0.25 % glucose solution, the content of PHB increased, after an initial lag, gradually up to the 10th day. After 1-d cultivation the content of PHB in the batch system increased even in the presence of diammonium hydrogen phosphate. In a heterocontinuous system no PHB accumulation took place in the presence of this source of nitrogen and phosphorus as long as the C:N ratio of theadded substrate was 10: 1.


Batch System Repeated Addition Crotonic Acid Coryneform Bacterium Hydroxybutyric Acid 


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1984

Authors and Affiliations

  • A. Hanzlíkova
    • 1
  • A. Jandera
    • 1
  • F. Kunc
    • 1
  1. 1.Department of Microbial Ecology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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