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Dynamics of changes in specific hypersensitivity and of the nonspecific increase in sensitivity to endotoxin in BCG-sensitized mice

Динамика изменений специфической гиперсенситивности и неспецифического повышеиня чувствительности к эндотоксину у сенсибилизированных BCG мышей

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Summary

  1. (1)

    Quantitative changes in specific hypersensitivity and nonspecific sensitivity were studied at different intervals after sensitization in white mice sensitized with different doses of BCG vaccine.

  2. (2)

    Specific hypersensitivity was determined by the pad test, from the degree of swelling of the pad after the injection of 10 μg. PPD. Nonspecific sensitivity was determined from the percentage of deaths among mice to which 0.1 mg.Salmonella paratyphi B endotoxin was administered intravenously.

  3. (3)

    It was found that specific hypersensitivity reached maximum values two to four months after sensitization. Maximum nonspecific sensitivity to the effect of endotoxin occurred two to three weeks after sensitization. The size of the sensitization dose was directly proportionale to the increase in nonspecific sensitivity and was not directly related to the degree of specific hypersensitivity.

  4. (4)

    These results exclude the possibility that nonspecific sensitivity to endotoxin develops directly as a causal consequence of the delayed type of hypersensitivity and that it is one of the manifestations of this hypersensitivity. The findings are in agreement with the hypothesis that the change in sensitivity to endotoxin is the outcome of the summation of toxic stimuli formed during the hypersensitivity reaction of the cells with antigenin vivo.

Abstract

  1. (1)

    На различных сроках после сенсибилизапии белых мышей различными дозами вакпины BCG исследовались количествнные измения спепифической гиперсенситивности, а также изменения неспецифической чувствительности.

  2. (2)

    Специфическую гиперсенситивность определяли «пробой на лапке»—по отечности лапки после впрыскивания 10 μг PPD. Неспепифическую чувствительность определяли на основании процента гибели мышей после внутривенного впрыскивания 0,1 мг эндотоксина S. paratyphi B.

  3. (3)

    Было установлено, что наиболее высоких показателей спепифическая гиперсенситивность достигает в период 2–4 месяцев поле сенсибилизации. Повышенная неспецифическая восприимчивость к действию эндотоксина оказывается максимальной через 2–3 недели после сенсибилизации. Величина сенсибилизирующей дозы прямо пропорциональна повышению неспецифической восприимчивости, но не находится в прямой зависимости от степени специфической гиперсенситивности.

  4. (4)

    Эти данные исключают возможность того, чтобы неспецифическая чувствительность К эндотоксину возникала прямо в причинной зависимости от гиперсенситивности позднего типа и была одним из проявлений зтой гиперсенситивности. Полученные нами результаты отвечают гипотезе, что изменения чувствительности к эндотоксину развиваются в результате суммации токсических раздражений, возникающих при взаимодействии гиперсенситивных клеток с антигеном in vivo.

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Authors and Affiliations

  1. Institute of Sera and Vaccines, Prague 12

    O. Vejbora, J. Johanovský & M. Vrána

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  1. O. Vejbora
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  2. J. Johanovský
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  3. M. Vrána
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Vejbora, O., Johanovský, J. & Vrána, M. Dynamics of changes in specific hypersensitivity and of the nonspecific increase in sensitivity to endotoxin in BCG-sensitized mice. Folia Microbiol 6, 370–378 (1961). https://doi.org/10.1007/BF02877178

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