Folia Microbiologica

, Volume 27, Issue 4, pp 242–244 | Cite as

Long-term preservation of yeast cultures in liquid nitrogen

  • Z. Hubálek
  • A. Kocková-Kratochvílová


Nineteen strains of taxonomieally diverse yeast species tested survived freezing and subsequent five-year storage in liquid nitrogen at ™196 °C, using a medium M 2 composed of malt extract, yeast extract, peptone, calf serum and dimethyl sulfoxide. Viability of the yeast cultures after long-term storage ranged from 5 to 97 % (average 62 %) compared with the viability of the cultures prior to freezing. The use of liquid nitrogen refrigeration for preserving yeast cultures is strongly advocated.


Malt Extract Yeast Culture Agar Slant Brewing Yeast Nitrogen Freezing 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1982

Authors and Affiliations

  • Z. Hubálek
    • 1
  • A. Kocková-Kratochvílová
    • 2
  1. 1.Institute of ParasitologyCzechoslovak Academy of SciencesPrague
  2. 2.Institute of ChemistrySlovak Academy of SciencesBratislava

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