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Possibilities of the conjugation process in mycobacteria

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Abstract

Results obtained when studying conjugation in mycobacteria by means of different methods are summarized. The method of conjugation on surface of a solid complete medium was tested with different auxotrophic mutants of different strains ofMycobacterium smegmatis. It was not possible to obtain positive results even by means of the above method. This was probably due to unsuitability of the chosen strains ofMycobacterium smegmatis. Preparation of the donor strain by transfer of the F factor fromEscherichia coli F’ORF 1ade + lac+ pro+ toMycobacterium phlei PA adeStm r by means of sexduction is described. Frequency of the phenotype PAade + Stmr increased in the average by two and a half orders of magnitude with respect to the control, however, a further transfer from cultures of the cellsade + Stmr to cells ade could not be demonstrated. Experiments aimed at transferring the R factor from strainsEscherichia coli K-12 toMycobacterium phlei were unsuccessful.

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Abbreviations

Cyc:

oycloserine

Nal:

nalidixic acid

Stm:

streptomycin

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Authors and Affiliations

  1. Department of Bacterial Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    J. Koníček & M. Koníčková-Radochová

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  1. J. Koníček
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  2. M. Koníčková-Radochová
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Koníček, J., Koníčková-Radochová, M. Possibilities of the conjugation process in mycobacteria. Folia Microbiol 20, 382–388 (1975). https://doi.org/10.1007/BF02877039

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