Death ofLactobacillus acidophilus caused by incorporated3H-thymine during incubation without amino acids
Of the cells ofLactobacillus acidophilus R-26 incorporated3H-thymine (specific radioactivity 1.57 Ci/mmol or 3.15 Ci/mmol), their transfer to a medium without essential amino acids resulted in their death. This death may be interpreted in such a way that cell damage caused by disintegration of tritium cannot be effectively repaired under conditions of amino acid deprivation. The experimental conditions make it possible to explain this death either as a result of inhibition of protein or RNA synthesis or as a result of the absence of amino acids. These possibilities were tested in experiments, in which the synthesis of proteins and RNA was inhibited by specific inhibitors in the presence of amino acids. Under these conditions no death of cells was detected, thus indicating that free amino acids play a role in the repair of radiation damage.
KeywordsFolic Acid Tritium Free Amino Acid Thymine Radiation Damage
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
- AA medium
medium without amino acids
Apelgot S., Latarjet R.
: Marquage d’un acide déoxyribonucleéique bacterienne par le radiophosphore, le radiocarbone et le tritium: Comparison des effects letaux.Biochim. Biophys. Acta
, 40 (1962).PubMedCrossRefGoogle Scholar
Cleaver J. E., Thomas G. H., Burki H. J.
: Biological damage from intranuclear tritium: DNA strand breaks and their repair.Science
, 996 (1972).PubMedCrossRefGoogle Scholar
Feinendegen J. E.
: Tritium labelled molecules in biology and medicine. Academic Press, New York (1967).Google Scholar
: DNA synthesis in Lactobacillus acidophilus in the absence of RNA and protein synthesis: A study with rifampicin.Molec. Gen. Genetics
, 140 (1969).CrossRefGoogle Scholar
Koukalová B., Reich J.
: Stringent control of RNA synthesis inLactobacillus acidophilus
. Folia Microbiol.13
, 20 (1968).CrossRefGoogle Scholar
Koukalová B., Soška J., Reich J.
: Inhibition of thymine synthesis in Lactobacillus acidophilus R-26 by deoxyadenosine-5′-monophosphate.Ant. van Leeuwenhoek J. Microbiol. Serol.
, 17 (1967).CrossRefGoogle Scholar
: Lethal and mutagenic effects of tritium decay produced by tritiated compounds incorporated into bacteria and bacteriophages. Biological effects of transmutation and decay of incorporated radio-isotopes. Proceedings of a panel, Vienna (1967).Google Scholar
Person S., Lewis H. L.
: Effects of decay of incorporated3
H-thymidine on bacteria.Biophys. J.
, 451 (1962).PubMedGoogle Scholar
Reich J., Soška J.
: Thymineless death inLactobacillus acidophilus
, 40 (1970).CrossRefGoogle Scholar
Rosenthal P. N., Fox M. S.
: Effects of disintegration of incorporated3
P on the physical and biological properties of DNA.J. Mol. Biol.
, 441 (1970).PubMedCrossRefGoogle Scholar
,: Growth ofLactobacillus acidophilus
in the absence of folic acid.J. Bacteriol.
, 1849 (1966).Google Scholar
Soška J., Kuhrová V., Koukalová B.
: Effect of rifampicin and free amino acids on replication of DNA inLactobacillus acidophilus
, 358 (1974).CrossRefGoogle Scholar
© Institute of Microbiology, Academy of Sciences of the Czech Republic and Springer Dordrecht 1976