Folia Microbiologica

, Volume 21, Issue 2, pp 107–116 | Cite as

Death ofLactobacillus acidophilus caused by incorporated3H-thymine during incubation without amino acids

  • B. Koukalová


Of the cells ofLactobacillus acidophilus R-26 incorporated3H-thymine (specific radioactivity 1.57 Ci/mmol or 3.15 Ci/mmol), their transfer to a medium without essential amino acids resulted in their death. This death may be interpreted in such a way that cell damage caused by disintegration of tritium cannot be effectively repaired under conditions of amino acid deprivation. The experimental conditions make it possible to explain this death either as a result of inhibition of protein or RNA synthesis or as a result of the absence of amino acids. These possibilities were tested in experiments, in which the synthesis of proteins and RNA was inhibited by specific inhibitors in the presence of amino acids. Under these conditions no death of cells was detected, thus indicating that free amino acids play a role in the repair of radiation damage.


Folic Acid Tritium Free Amino Acid Thymine Radiation Damage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





amino acids

AA medium

medium without amino acids






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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic and Springer Dordrecht 1976

Authors and Affiliations

  • B. Koukalová
    • 1
  1. 1.Institute of BiophysicsCzechoslovak Academy of SciencesBrno

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