Advertisement

Folia Microbiologica

, 21:355 | Cite as

Antibiotic-induced changes of mycelial growth ofBotrytis cinerea

  • H. Baráthová
  • V. Betina
Article

Abstract

The effect of antibiotics and metabolic inhibitors on mycelial growth ofBotrytis cinerea was followed. Inhibitors of protein synthesis, chloramphenicol, erythromycin and tetracycline inhibit growth or sporulation ofBotrytis cinerea. Ethidium bromide, 5-fluorouracil, phenylethylalcohol and K 20 cause granulation, vacuolization and undulation of hyphae. 2,4-Dinitrophenol, boromycin, macrotetrolides, monensin, scopathricin and TX2 at subfungistatic concentrations induce intensive branching of hyphal tipsi.e. at the site of synthesis of the cell wall. In older hyphae grown in the absence of the antibiotics the branching begins after their addition, particularly in the septum region. When comparing the results referred to here with those obtained previously and on the basis of literature data it may be assumed that the changes in polarity of growth ofBotrytis cinerea might be caused primarily or secondarily by impairing membrane functions and formation of cell walls.

Keywords

Cytochalasin Griseofulvin Monensin Citrinin Validamycin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Banerjee S., Margulis L.: Reversible inhibition of cilia regeneration inStentor coeruleua by isopropyl-n-phenyl carbamate.Nature, (London) 224, 180 (1969).CrossRefGoogle Scholar
  2. Banerjee S., Margulis L.: Inhibition of cilia regeneration by neoplastic agents.Cancer Chemotherapy Reports 55, 531 (1971).Google Scholar
  3. Baráth Z., Babáthová H., Betina V., Nemec P.: Production and release of riboflavin from the myceliumEremothecium ashbyi in the presence of cyanein and amphotericin B. (In Slovak)Biológia (Bratislava) 24, 684 (1969).Google Scholar
  4. Baráth Z., Babáthová H., Betina V., Nemec P.: Ramihyphins-antifungal and morphogenetic antibiotics fromFusarium sp. S-435.Folia Microbiol. 19, 507 (1974).Google Scholar
  5. Baráthová H., Betina V., Nemec P.: Morphological changes induced in fungi by antibiotics.Folia Microbiol. 14, 475 (1969).Google Scholar
  6. Baráthová H., Betina V., Baráth Z., Nemec P.: Morphogenic effects of ramihyphin A in filamentous fungi.Folia Microbiol. 20, 97 (1975).CrossRefGoogle Scholar
  7. Betina V., Babáthová H.: Citrinin-an inducer of permeability changes inEremothecium ashbyi.J. Antibiot. Ser. A 21, 628 (1968).Google Scholar
  8. Betina V., Mičeková D.: Antimicrobial properties of fungal macrolide antibiotics.Zeitsch. Allg. Mikrobiol. 12, 355 (1972).CrossRefGoogle Scholar
  9. Betina V., Rúčková E.: Effect of citrinin, desertomycin and of polyene antibiotics on plant and animal cell membranes.Biológia (Bratislava) 27, 463 (1972).Google Scholar
  10. Betina V., Mičeková D.: Morphogenetio activity of cytochalasins, cyanein and monorden inBotrytis cinerea.Zeitsch. Allg. Mikrobiol. 13, 287 (1973).CrossRefGoogle Scholar
  11. Betina V., Droubet E., Segretain G.: Action de la cyanéine invitro sur des champignons pathogènes.Ann. Inst. Pasteur 109, 933 (1965).Google Scholar
  12. Betina V., Betinová M., Kutková M.: Effects of cyanein on growth and morphology of pathogenic fungi.Arch. Mikrobiol. 55, 1 (1966).PubMedCrossRefGoogle Scholar
  13. Betina V., Baráthová H., Nemec P., Baráth Z.: Permeability changes caused by desertomycin in fungal, animal and plant cells.J. Antibiot. 22, 129 (1969).PubMedGoogle Scholar
  14. Betina V., Janstová D., Spišiaková J.: Effect of antibiotics on the life cycle ofNeurospora crassa.Folia Microbiol. 20, 340 (1975).Google Scholar
  15. Betina V., Mičeková D., Nemec P.: Antimicrobial properties of cytochalasina and their alteration of fungal morphologyJ. Gen. Microbiol. 71, 343 (1972).Google Scholar
  16. Brian P. W., Curtis P. J., Hemming H. G.: A substance causing abnormal development of fungal hyphae produced byPenicillium janczewskii.Trans. Brit. Mycol. Soc. 29, 173 (1946).CrossRefGoogle Scholar
  17. Esuruoso O. F., Price T. V., Wood R. K. S.: Germination ofBotrytis cinerea conidia in the presence of quintozene, teonazine and dicloran.Trans. Brit. Mycol. Soc. 51, 405 (1968).Google Scholar
  18. Fuska J., Kuhr I., Nemec P., Fusková A.: Antitumor antibiotic produced byPenicillium stipitatum Thorn.J. Antibiot. 27, 123 (1974).PubMedGoogle Scholar
  19. Fuska J., Sláviková E., Adámková M.: Secondary metabolites ofP. stipitatum Thom. I. Compounds of tropolone character. (In Slovak)Biológia (Bratislava),30, 669 (1975).Google Scholar
  20. Gale E. F., Cundliffe E., Reynolds P. E., Richmond M. H., Waking M. J.: The molecular basis of antibiotic action. John Wiley and Sons, London, pp. 147, 151 (1972).Google Scholar
  21. Henderson P. J. F., Lardy H. A.: Bongkrekic acid. An inhibitor of the adenine nucleotic translocase of mitochondria.J. Biol. Ghem. 245, 1319 (1970).Google Scholar
  22. Holík M., Kuhr I.: Determination of structure of stipitalide: a new tropolone fromPenicillium stipitatum Thom.Ghem. Commun. 1973, 65 (1973)CrossRefGoogle Scholar
  23. Huber F. M.:Griseofulin. In: Gottlieb, D., Shaw P. D. (Editors), Antibiotics, Volume I, Mechanism of Action. Springer-Verlag, Berlin, p. 181–189 (1967).Google Scholar
  24. Hütter R., Keller-Schierlein W., Nüesch J., Zähner H.: Scopamycine.Arch. Mikrobiol. 51, 1 (1965).CrossRefGoogle Scholar
  25. Iwasa I., Higashide E., Yamamoto H., Shibata M.: Studies on validamycins, new antibiotics. II. Production and biological properties of validamycins A and B.J. Antibiot. 24, 107 (1971).PubMedGoogle Scholar
  26. Katz D., Rosenberg R. F.: Hyphal wall synthesis inAspergillus nidulans: effect of protein synthesis inhibition and osmotic shock on chitin insertion and morphogenesis.J. Bacteriol. 108, 184 (1971).PubMedGoogle Scholar
  27. Katz D., Goldstein D., Rosenberger R. F.: Model for branch initiation inAspergillus nidulans based on measurements of growth parameters.J. Bacteriol. 109, 1097 (1972).PubMedGoogle Scholar
  28. Lysek G.: Zonierungen und Hexenringe Morphologische Differezierungen bei Pilzen.Naturwiss. Rundschau 27, 449 (1974).Google Scholar
  29. Malawista S. E., Sato H., Bensch K. G.: Vinblastine and griseofulvin reversibly disrupt the living mitotic spindle.Science (Washington) 160, 770 (1968).CrossRefGoogle Scholar
  30. Mizel S. B., Wilson L.: Inhibition of the transport of several hexoses in mammalian cells by cytochalasinJ. Bid. Ghem. 247, 4102 (1972).Google Scholar
  31. Nioh T., Mizushima S.: Effect of validamycin on the growth and morphology ofPellicularia sasakii.J. Gen. Appl. Microbiol. 20, 373 (1974).CrossRefGoogle Scholar
  32. Oliver P. T. P.: Influence of cytochalasin B on hvphal morphogenesis ofAspergillus nidulans.Protoplasma 76, 279 (1973).PubMedCrossRefGoogle Scholar
  33. Pache W.:Boromycin. In: Corcoran J. W., Hahn F. E.: Antibiotics III. Berlin-Heidelberg-New York, Springer, p. 585 (1975).Google Scholar
  34. Pache W., Zähner H.: Studies on the mechanism of action of boromycin.Arch. Mikrobiol. 67, 156 (1969).PubMedCrossRefGoogle Scholar
  35. Richmond D. V., Pring R. J.: The effect of benomyl on the fine structure ofBotrytis foboe.J. Gen. Microbiol. 66, 79 (1971).Google Scholar
  36. Šašek V., Musílek V.: Effects of the new antifungal antibiotic mucidin II. Morphological changes brought about in sensitive test microorganisms.Folia Microbiol. 19, 142 (1974).CrossRefGoogle Scholar
  37. Šubík J., Behúň M.: Effect of bongkrekic acid on growth and metabolism of filamentous fungi.Arch. Microbiol. 97, 81 (1974).CrossRefGoogle Scholar
  38. Šubík J., Kolarov J., Kováč L.: Obligatory requirement of mitochondrial ATP for normal functioning of the eucaryotic cell.Biochem. Biophys. Res. Commun. 49, 192 (1972).PubMedCrossRefGoogle Scholar
  39. Šubík J., Behúň M., Šmigáň P. Musílek V.: Mode of action of mucidin a new antifungal antibiotic produced by the basidiomyceteOudemansiella mucida.Biochim. Biophys. Acta 343, 363 (1974).PubMedGoogle Scholar
  40. Wessels N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamadak M.: Microfilaments, in cellular and development processes.Science, (Washington)17, 135 (1971).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1976

Authors and Affiliations

  • H. Baráthová
    • 1
  • V. Betina
    • 1
  1. 1.Department of Technical Microbiology and Biochemistry, Faculty of ChemistrySlovak Polytechnical UniversityBratislava

Personalised recommendations