Archives of Pharmacal Research

, Volume 14, Issue 3, pp 231–236 | Cite as

Solubilization of an angiotensin II binding site from rat liver

  • Sung-Hyun Chung
  • Ravi Iyengar
Original Articles


The high affinity binding sites for angiotensin II were solubilized from rat liver membranes by treatment with CHAPS. The binding protein was also partially purified by angiotensin III inhibitor-coupled Affi-gel affinity chromatography. Binding to the intact membranes as well as to the solubilized preparation was specific and saturable. According to the Scatchard plot, the membrane preparations exhibited a single class of high affinity binding sites with aKd of 0.71 nM. The solubilized preparation also showed the presence of a single class of binding sites with less affinity (Kd of 14 nM). Meanwhile the competition studies using angiotensin II analogues represented two separate binding sites for angiotensin II and single binding site for antagonist. These latter findings were correlated to the results provided by Garrison's research group. More works are needed to clarify this discrepancy.


Renin-Angiotensin system angiotensin II receptor (binding site) solubilization affinity chromatography 


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Copyright information

© The Pharmaceutical Society of Korea 1991

Authors and Affiliations

  • Sung-Hyun Chung
    • 1
  • Ravi Iyengar
    • 2
  1. 1.College of PharmacyKyung Hee UniversitySeoulKorea
  2. 2.Department of PharmacologyMount Sinai School of Medicine of the City University of New YorkNew York

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