Activity of Mg2+-ATPase and cytochrome c-Oxidase in microsporocytes and anther wall during microsporogenesis inLarix europaea D.C.
Studies have been made on the activity of two mitochondrial enzymes, Mg2+ ATPase (E.C.184.108.40.206.) and cytochrome c-oxidase (E.C.I.9.3.2.) in microsporocytes and somatic cells of anther in larch.
The material for study were homogeneous fractions of microsporocytes from 15 stages of meiosis and the attendant anther somatic cells. The results have demonstrated that cells undergoing meiosis exhibit considerable mitochondrial metabolic activity. It is characterized by considerable variations in the activity level of both enzymes studied. The level and dynamics of variations of Mg2+-ATPase and cytochrome c-oxidase activity in microsporocytes are clearly different from those in the anther somatic cells. The cytochrome c-oxidase activity in microsporocytes throughout microsporogenesis is higher compared with that in the anther wall cells, whereas the Mg2+-ATPase activity in microsporocytes averagesca. one half that in the anther somatic cells
The dynamics of activity variations of the enzymes under study suggests enhanced mitochondrial metabolism in the period of middle diplotene and young dyad. This result supports the thesis following from our earlier studies that the middle diplotene and young dyad constitute specific metabolic switches in microsporogenesis in larch.
KeywordsATPase Activity Successive Stage Mitochondrial Enzyme Anther Wall Tetrad Stage
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- Chwirot, W. B.: The method of acquirement of fractions of microsporoeytes in successive stages of meiosis inLarix europaea L. Acta Physiol. Plant.2: 103–112, 1980.Google Scholar
- Chwirot, W. B., Górska-Brylass, A.: Variations of total protein content and protein synthesis during microsporogenesis inLarix europaea D.C. Acta Soc. Bot. Pol.50: 33–38, 1981.Google Scholar
- Dickinson, H. G.: Cytoplasmic differentiation during microsporogenesis in higher plants. Acta Soc. Bot. Pol.50: 3–12, 1981.Google Scholar
- Fiske, C. H., Subbarow, Y.: The colorimetric determination of phosphorus. J. biol. Cliem.66: 375–400, 1925.Google Scholar
- Górska-Brylass, A., Chwirot, W. B., Majewska, A.: Ultrastructural and metabolic transformations of the larch microspore during Gi period of tlio post-meiotic interphase. Postepy Biol. Komórki11: 577–580, 1984.Google Scholar
- Górska-Brylass A., Chwirot, W. B., Michalski, L.: IAA-peroxidase relation in the microsporocytes and anther wall during successive stages of meiosis inLarix europaea D.C. Acta Soc Bot. Pol.50: 67–74, 1981.Google Scholar
- Heslop-Harrison, J.: The cytoplasm and its organelles during meiosis. In:Heslop-Harrison, J.: Pollen-Development and Physiology. Pp. 16–32. Butterworths, London 1971.Google Scholar
- Luck, B. T., Jordan, E. G.: The mitochondria and plastkls during microsporogenesis inHyacinthoides non-scripta (L.)Chouard. Ann. Bot.45: 511–514, 1980.Google Scholar
- Maruyama, K.:Electron microscopic observation of plastids and mitochondria during pollen development inTradescantia paludosa. Cytologia33: 482–497, 1968.Google Scholar
- Reznickova, S. A., Willemse, M. T. M.: Formation of pollen in the anther ofLilium. II. The function of the surrounding tissues in the formation of pollen and pollen wall. Acta bot. neerl.29: 141–156. 1980.Google Scholar
- Suetfeld, R., Wiermann, R.: Die Entwicklung zweier o-Dihydroxyphenol O-Methyltransferasen waehrend der Mikrosporogenese vonTulipa cv. Apeldoorn. Z. Pflanzenphysiol.97: 283 to 288, 1980.Google Scholar
- Willemse, M. T. M.: Morphological and quantitative changes in the population of cell organelles during microsporogenesis ofPinus silvestris L. III. Morphological changes during the tetrad stage and the young microspore. A quantitative approach to the changes in the population of cell organelles. Acta bot. neerl.20: 498–523, 1971.Google Scholar