Biologia Plantarum

, Volume 29, Issue 3, pp 167–174 | Cite as

Activity of Mg2+-ATPase and cytochrome c-Oxidase in microsporocytes and anther wall during microsporogenesis inLarix europaea D.C.

  • Wieslawa B. Chwirot
  • Alicja Górska-brylass


Studies have been made on the activity of two mitochondrial enzymes, Mg2+ ATPase (E.C. and cytochrome c-oxidase (E.C.I.9.3.2.) in microsporocytes and somatic cells of anther in larch.

The material for study were homogeneous fractions of microsporocytes from 15 stages of meiosis and the attendant anther somatic cells. The results have demonstrated that cells undergoing meiosis exhibit considerable mitochondrial metabolic activity. It is characterized by considerable variations in the activity level of both enzymes studied. The level and dynamics of variations of Mg2+-ATPase and cytochrome c-oxidase activity in microsporocytes are clearly different from those in the anther somatic cells. The cytochrome c-oxidase activity in microsporocytes throughout microsporogenesis is higher compared with that in the anther wall cells, whereas the Mg2+-ATPase activity in microsporocytes averagesca. one half that in the anther somatic cells

The dynamics of activity variations of the enzymes under study suggests enhanced mitochondrial metabolism in the period of middle diplotene and young dyad. This result supports the thesis following from our earlier studies that the middle diplotene and young dyad constitute specific metabolic switches in microsporogenesis in larch.


ATPase Activity Successive Stage Mitochondrial Enzyme Anther Wall Tetrad Stage 
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© Academia 1987

Authors and Affiliations

  • Wieslawa B. Chwirot
    • 1
  • Alicja Górska-brylass
    • 1
  1. 1.Department of Plant Cytology and Genetics, Institute of BiologyCopernicus UniversityToruńPoland

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