Abstract
Studies have been made on the activity of two mitochondrial enzymes, Mg2+ ATPase (E.C.3.6.1.3.) and cytochrome c-oxidase (E.C.I.9.3.2.) in microsporocytes and somatic cells of anther in larch.
The material for study were homogeneous fractions of microsporocytes from 15 stages of meiosis and the attendant anther somatic cells. The results have demonstrated that cells undergoing meiosis exhibit considerable mitochondrial metabolic activity. It is characterized by considerable variations in the activity level of both enzymes studied. The level and dynamics of variations of Mg2+-ATPase and cytochrome c-oxidase activity in microsporocytes are clearly different from those in the anther somatic cells. The cytochrome c-oxidase activity in microsporocytes throughout microsporogenesis is higher compared with that in the anther wall cells, whereas the Mg2+-ATPase activity in microsporocytes averagesca. one half that in the anther somatic cells
The dynamics of activity variations of the enzymes under study suggests enhanced mitochondrial metabolism in the period of middle diplotene and young dyad. This result supports the thesis following from our earlier studies that the middle diplotene and young dyad constitute specific metabolic switches in microsporogenesis in larch.
Similar content being viewed by others
References
Bird, J., Porter, K. K., Dickinson, H. G.: Events in the cytoplasm during male meiosis inLilium. J. Cell Sci.59: 27–42, 1983.
Chwirot, W. B.: The method of acquirement of fractions of microsporoeytes in successive stages of meiosis inLarix europaea L. Acta Physiol. Plant.2: 103–112, 1980.
Chwirot, W. B., Górska-Brylass, A.: Variations of total protein content and protein synthesis during microsporogenesis inLarix europaea D.C. Acta Soc. Bot. Pol.50: 33–38, 1981.
Dickinson, H. G.: Cytoplasmic differentiation during microsporogenesis in higher plants. Acta Soc. Bot. Pol.50: 3–12, 1981.
Fiske, C. H., Subbarow, Y.: The colorimetric determination of phosphorus. J. biol. Cliem.66: 375–400, 1925.
Górska-Brylass, A., Chwirot, W. B., Majewska, A.: Ultrastructural and metabolic transformations of the larch microspore during Gi period of tlio post-meiotic interphase. Postepy Biol. Komórki11: 577–580, 1984.
Górska-Brylass A., Chwirot, W. B., Michalski, L.: IAA-peroxidase relation in the microsporocytes and anther wall during successive stages of meiosis inLarix europaea D.C. Acta Soc Bot. Pol.50: 67–74, 1981.
Heslop-Harrison, J.: The cytoplasm and its organelles during meiosis. In:Heslop-Harrison, J.: Pollen-Development and Physiology. Pp. 16–32. Butterworths, London 1971.
Hodges, T. K., Leonard, R. T.: Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. Methods Enzymol.32: 392–406, 1974.
Hotta, Y., Stern, H.: Synthesis of messenger-like ribonucleic acid and protein during meiosis in isolated cells ofTrilium erectum. J. Cell Biol.19: 45–57, 1963.
Leonard, R. T., Hodges, T. K.: Characterization of plasma membrane associated ATPase activity of oat roots. Plant Physiol.52: 6–12, 1973.
Linskens, H. F.: Die Änderung des Protein und Enzym-Musters während der Pollenmeiose und Pollenentwicklung. Physiologische Untersuchungen zur Reifeteilung. Planta69: 79–91, 1966.
Lowry, J. O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193: 265–275, 1951.
Luck, B. T., Jordan, E. G.: The mitochondria and plastkls during microsporogenesis inHyacinthoides non-scripta (L.)Chouard. Ann. Bot.45: 511–514, 1980.
Maruyama, K.:Electron microscopic observation of plastids and mitochondria during pollen development inTradescantia paludosa. Cytologia33: 482–497, 1968.
Pierce, W. S., Hendrix, D. L.: Utilization of enzyme markers to determine the location of plasma membrane fromPisum epicotyls on sucrose gradients. Planta146: 161–169, 1979.
Reznickova, S. A., Willemse, M. T. M.: Formation of pollen in the anther ofLilium. II. The function of the surrounding tissues in the formation of pollen and pollen wall. Acta bot. neerl.29: 141–156. 1980.
Sperk, G., Tuppy, H.: Differences between adenosine triphosphatasos from monocotyledonous and dicotyledonous plants. Plants Physiol.59: 155–157, 1977.
Suetfeld, R., Wiermann, R.: Die Entwicklung zweier o-Dihydroxyphenol O-Methyltransferasen waehrend der Mikrosporogenese vonTulipa cv. Apeldoorn. Z. Pflanzenphysiol.97: 283 to 288, 1980.
Willemse, M. T. M.: Morphological and quantitative changes in the population of cell organelles during microsporogenesis ofPinus silvestris L. III. Morphological changes during the tetrad stage and the young microspore. A quantitative approach to the changes in the population of cell organelles. Acta bot. neerl.20: 498–523, 1971.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chwirot, W.B., Górska-brylass, A. Activity of Mg2+-ATPase and cytochrome c-Oxidase in microsporocytes and anther wall during microsporogenesis inLarix europaea D.C.. Biol Plant 29, 167–174 (1987). https://doi.org/10.1007/BF02876822
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02876822