Folia Microbiologica

, Volume 20, Issue 2, pp 124–129 | Cite as

Effect of boseimycin on some enzyme systems ofBacillus subtilis

  • T. K. Mishea


The effect of boseimycin on thein vitro activity andin vivo synthesis of alkaline phosphatase, aconitase and lactate, isocitrate, glutamate and alanine dehydrogenases was studied inBacillus subtilis. At a subinhibitory concentration, synthesis of glutamate dehydrogenase was stimulated but alkaline phosphatase, lactate dehydrogenase and aconitase synthesis was inhibited. On the contrary, boseimycin inhibited slightly the activity of lactate dehydrogenase in cell-free extracts. Glutamate dehydrogenase and aconitase activities were not affected.


Bacillus Subtilis Sodium Azide Isocitrate Glutamate Dehydrogenase Subinhibitory Concentration 
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  1. Anfinsen C. B.: Aconitase from pig heart muscle.Methods in Enzymol. Acad. Press Inc., N.Y. 1, 695 (1955).Google Scholar
  2. Coleman G., Elliot W. H.: Stimulation of extracellular ribonuclease formation inBacillus subtilis by actinomycin D.Nature 202, 1083 (1964).PubMedCrossRefGoogle Scholar
  3. Coleman G., Elliot W. H.: Extracellular ribonuclease formation inBacillus subtilis and its stimulation by actinomycin D.Biochem. J. 95, 699 (1965).PubMedGoogle Scholar
  4. Kadowaki K., Yamaguchi K., Mauro B.: Effect of actinomycin D on protein, ribonucleic acid and enzyme formation inBacillus subtilis.J. Gen. Appl. Microbiol. 12, 157 (1966).CrossRefGoogle Scholar
  5. Korstberg A.: Lactic dehydrogenase of muscle.Methods in Enzymol. Acad. Pres. Inc. N.Y. 1, 441 (1955).Google Scholar
  6. Lowry O. H., Rosebrough N. J., Farra L., Randal R. J.: Protein measurement with Folin phenol reagent.J. Biol. Chem. 193, 265 (1951).PubMedGoogle Scholar
  7. Majumdar S. K., Bose S. K.: Mycobacillin, a new antifungal antibiotic produced byBacillus subtilis.Nature 181, 134 (1958).PubMedCrossRefGoogle Scholar
  8. Mandal A., Majumdar S. K.: Effect of streptomycin on some enzyme systems ofBacillus subtilis.Appl. Microbiol. 16, 1786 (1968).PubMedGoogle Scholar
  9. Mishra T. K.: Comparative studies on the effects of boseimycin and streptomycin inBacillus subtilis.Gurr. Sci. 40, 440 (1971).Google Scholar
  10. Mishra T. K., Mandi P.: Effects of boseimycin on different cellular processes inBacillus subtilis. Symp. “Control Mechanisms in Cellular Processes” B.A.R.C., Govt. of India, Bombay, pp. 615, 1973.Google Scholar
  11. Mishra T. K., Sinha R. K.: Mechanism of action of boseimycin.Experientia 27, 642 (1971).CrossRefGoogle Scholar
  12. Ochoa S.: Isocitric dehydrogenase system (TPN) from pig heart.Methods in Enzymol. Acad. Press Inc., N.Y. 1, 699 (1955).Google Scholar
  13. Pollock M. R.: Penicillinase synthesis. The differential effect of actinomycin D on the biosynthesis of enzymes inBacillus subtilis andBacillus cereus.Biochem. Biophys. Acta 76, 80 (1963).PubMedCrossRefGoogle Scholar
  14. Sinha R. K.: Boseimycin - A new streptothricin-like antibiotic.J. Antibiot. 23, 360 (1970).PubMedGoogle Scholar
  15. Sinha R. K., Nandi P. N.: A new antibacterial agent produced byStreptomyces sp. Ac6 569.Experientia 24, 795 (1968).PubMedCrossRefGoogle Scholar
  16. Steecker H. J.: L-Glutamic dehydrogenase from liver.Methods in Enzymol. Acad. Press Inc., N.Y. 2, 220 (1955).Google Scholar
  17. Sypherd P. S., Strauss N.: Chloramphenicol-promoted repression of β-galactosidase synthesis inEscherichia coli.Proc. Natl. Acad. Sci., U.S. 49, 400 (1963).CrossRefGoogle Scholar
  18. Zimmer C. H., Triebel H., Thrun H.: Interaction of streptothricin and related antibiotics with nucleic acids.Biochem. Biophys. Acta 145, 742 (1967).PubMedGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1975

Authors and Affiliations

  • T. K. Mishea
    • 1
  1. 1.Department of MicrobiologyBose InstituteCalcuttaIndia

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