Transformation of protoplasts of nontransformableBacillus subtilis mutants by plasmid pUB 110 DNA
Protoplasts rather than intact cells of nontransformableB. subtilis mutants were transformed by plasmid pUB 110 DNA. Transformability of protoplasts of the NT mutants indicates that the mechanism of uptake of the donor DNA by protoplasts differs from that by competent intact cells.
KeywordsTBAB Sodium Succinate High Frequency Transformation Medium Transformation Subtilis Mutant
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