Folia Microbiologica

, Volume 27, Issue 6, pp 465–467 | Cite as

Transformation of protoplasts of nontransformableBacillus subtilis mutants by plasmid pUB 110 DNA

  • Z. Jandová
  • P. Tichý
Brief Report


Protoplasts rather than intact cells of nontransformableB. subtilis mutants were transformed by plasmid pUB 110 DNA. Transformability of protoplasts of the NT mutants indicates that the mechanism of uptake of the donor DNA by protoplasts differs from that by competent intact cells.


TBAB Sodium Succinate High Frequency Transformation Medium Transformation Subtilis Mutant 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1982

Authors and Affiliations

  • Z. Jandová
    • 1
  • P. Tichý
    • 1
  1. 1.Department of Molecular Biology and Genetics, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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