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Folia Microbiologica

, Volume 23, Issue 5, pp 385–388 | Cite as

Biosynthesis of fatty acids and sterols in relation to the antibiotic formation inOudemansiella mucida

  • F. Nertjd
  • V. Musílek
Article

Abstract

The production of mucidin by the basidiomyceteOudemansiella mucida was negatively influenced by the application of D-glucitol as the main carbon source, the effect being independent of the growth rate of the mycelium. The rate of fatty acid synthesis was measured by incorporation of 1-14C-acetate. After 8 days of cultivation, the amount of fatty acids was approximately half that synthetized during cultivation on glucose. The specific rate of incorporation reached its maximum after seven days of cultivation. Incorporation of 2-14C-mevalonate into sterols was the same under the two sets of cultivation conditions. Acetate units from the degraded fatty acids are probably also utilized for antibiotic synthesis.

Keywords

Fatty Acid Synthesis Submerged Culture Main Carbon Source Label Acetate Glucitol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Dittmer J. C., Wells M. A.: Quantitative and qualitative analysis of lipids and lipid components, p. 482 to 530 in Colowick S. P., Kaplan N. O.:Methods in Enzymology. Vol. 14. Academic Press, New York 1969.Google Scholar
  2. Musílek V., Černá J., Šašek V., Semerdzieva M., Vondráček M.; Antifurigal antibiotic of the basidio myceteOudemansiella mucida. I. Isolation and cultivation of a producing strain.Folia Microbiol. 14, 377 (1969).CrossRefGoogle Scholar
  3. Nerud F., Musílek V.: Composition of lipids and production of mucidin in a submerged culture of the basidiomyceteOudemansiella mucida.Folia Microbiol. 21, 488 (1976).Google Scholar
  4. Vondráček M., Čapková J., Šleohta J., Benda A., Musílek V., Cudlín J.: The method of isolation of a new antifungal antibiotic.Czech. Pat. 136 495 (1970).Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1978

Authors and Affiliations

  • F. Nertjd
    • 1
  • V. Musílek
    • 1
  1. 1.Department of Experimental Mycology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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