Biosynthesis of fatty acids and sterols in relation to the antibiotic formation inOudemansiella mucida
- 15 Downloads
The production of mucidin by the basidiomyceteOudemansiella mucida was negatively influenced by the application of D-glucitol as the main carbon source, the effect being independent of the growth rate of the mycelium. The rate of fatty acid synthesis was measured by incorporation of 1-14C-acetate. After 8 days of cultivation, the amount of fatty acids was approximately half that synthetized during cultivation on glucose. The specific rate of incorporation reached its maximum after seven days of cultivation. Incorporation of 2-14C-mevalonate into sterols was the same under the two sets of cultivation conditions. Acetate units from the degraded fatty acids are probably also utilized for antibiotic synthesis.
KeywordsFatty Acid Synthesis Submerged Culture Main Carbon Source Label Acetate Glucitol
Unable to display preview. Download preview PDF.
- Dittmer J. C., Wells M. A.: Quantitative and qualitative analysis of lipids and lipid components, p. 482 to 530 in Colowick S. P., Kaplan N. O.:Methods in Enzymology. Vol. 14. Academic Press, New York 1969.Google Scholar
- Nerud F., Musílek V.: Composition of lipids and production of mucidin in a submerged culture of the basidiomyceteOudemansiella mucida.Folia Microbiol. 21, 488 (1976).Google Scholar
- Vondráček M., Čapková J., Šleohta J., Benda A., Musílek V., Cudlín J.: The method of isolation of a new antifungal antibiotic.Czech. Pat. 136 495 (1970).Google Scholar