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Folia Microbiologica

, Volume 17, Issue 3, pp 205–212 | Cite as

Esterases in mycobacteria

IV. The effect of macromolecular compounds isolated from a submerged culture ofMycobacterium phlei on the temporary activation of esterases
  • P. Procházka
  • M. Nohýnek
  • J. Rokos
Article

Abstract

Esterases ofMycobacterium phlei isolated by means of Sephadex G-100 chromatography could be temporarily activated by adding calcium ions. This activation could not be brought about in crude enzyme preparations from cells or in crude extracts from the culture filtrate. It was demonstrated that compounds (or a compound) without the esterase activity isolated after the separation of crude enzyme preparations on Sephadex G-100 (peak C) are responsible for the above difference. It was found that the suppression by compounds from the “C peak” of the temporary activation of esterases with calcium ions depends most probably on the ratio of these compounds to the quantity of the enzyme rather than on their concentration. In addition, the compounds of the C peak themselves were found to activate esterases. The activation was lower than the temporary activation with calcium ions. The possible mechanisms of the temporary activation of esterases and the importance of these findings, from the point of view of regulation of the activity of esterases during submerged cultivation ofMycobacterium phlei, are discussed.

Keywords

Crude Extract Culture Filtrate Temporary Activation Submerged Cultivation Crude Extract Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Procházka, P., Nohýnek M., Šroglová A., Rokos J.: Esterases in Mycobacteria. I. Carboxylic ester hydrolases in a submerged culture ofMycobacterium phlei.Folia Microbiol. 17, 17 (1972).CrossRefGoogle Scholar
  2. Procházka, P., Nohýnek M., Šroglová A., Rokos J.: Esterases in Mycobacteria. II. The isolation of carboxylic ester hydrolases from a submerged culture ofMycobacterium phlei.Folia Microbiol. 17, 28 (1972).CrossRefGoogle Scholar
  3. Procházka, P., Nohýnek M., Rokos J.: Esterases in Mycobacteria. III. A temporary activation of esterases ofMycobacterium phlei with ions of divalent metals.Folia Microbiol. 17, 196 (1972).CrossRefGoogle Scholar

Copyright information

© Academia, Publishing House of the Czechoslovak Academy of Sciences 1972

Authors and Affiliations

  • P. Procházka
    • 1
  • M. Nohýnek
    • 1
  • J. Rokos
    • 1
  1. 1.Department of General Microbiology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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