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Capillary electrophoretic separation of glycoproteins

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Abstract

A capillary electrophoretic approach has been developed for analyzing the microheterogeneity of glycoproteins. The key factors include the buffer composition, concentration, pH, and the surface treatment of the capillaries used. It has been shown that, if the buffer is mainly composed of alcohol amines and the capillaries are coated with polyacrylamide, ovalbumin from chicken egg can be separated into more than 25 peaks and that of transferrin about 10 peaks.

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Authors and Affiliations

  1. Institute of Chemistry, Chinese Academy of Sciences, 100080, Beijing, China

    Chen Yi

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  1. Chen Yi
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Project supported by the National Natural Science Foundation of China (Grant No. 29835020), by the Chinese Academy of Sciences (CAS) and by the Director Funds of the Institute of Chemistry of the CAS.

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Chen, Y. Capillary electrophoretic separation of glycoproteins. Sc. China Ser. B-Chem. 41, 71–76 (1998). https://doi.org/10.1007/BF02875560

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  • DOI: https://doi.org/10.1007/BF02875560

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