Science in China Series B: Chemistry

, Volume 42, Issue 2, pp 195–203 | Cite as

Enzyme-catalyzed reaction of voltammetric enzyme-linked immunoassay system based on OAP as substrate

  • Shusheng Zhang
  • Hongyuan Chen
  • Kui Jiao


Theo-aminophenol (OAP)-H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay new system has extremely high sensitivity. HRP can be measured with a detection limit of 6.0×1010 g/L and a linear range of 1.0×10-9-4.0×10-6 g/L. The pure product of H2O2 oxidizing OAP catalyzed by HRP was prepared with chemical method. The enzyme-catalyzed reaction has been investigated with electroanalytical chemistry, UV/Vis spectrum, IR spectrum,13C NMR,1H NMR, mass spectrum, elemental analysis, etc. Under the selected enzyme-catalyzed reaction conditions, the oxidation product of OAP with H2O2 catalyzed by HRP is 2-aminophenoxazine-3-one. The processes of the enzyme-catalyzed reaction and the electroreduction of the product of the enzymecatalyzed reaction have been described.


o-aminophenol horseradish peroxidase voltammetry enzyme-catalysis electrochemical immunoassay 


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Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Shusheng Zhang
    • 1
  • Hongyuan Chen
    • 1
  • Kui Jiao
    • 2
  1. 1.Department of ChemistryNanjing UniversityNanjingChina
  2. 2.Department of Applied ChemistryQingdao Institute of Chemical TechnologyQingdaoChina

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