Folia Microbiologica

, Volume 19, Issue 1, pp 71–78 | Cite as

The passive anaphylactic reaction in guinea pigs elicited by whole and cathepsin D-degraded antigen

  • J. Štefanovič
  • D. Kotulová
  • L’. Bergendi
  • I. Huzuláková


The systemic anaphylactic reaction and thein vitro anaphylactic contraction of the terminal segment of the ileum performed according to the Schultz-Dale technique, were elicited in guinea pigs passively sensitized with rabbit antibodies to human serum albumin, using whole and cathepsin D degraded antigen. The intensity of the systemic anaphylactic response that was evoked by degraded antigen was lower; a highly significant suppression of the response was obtained provided an antigen degraded more than by 70% was injected. With an increasing degradation of antigen, more animals responded with lower or even with no reaction; the number of animals developing severe or lethal shock, decreased at the same time. The number of animals that developed a medium anaphylactic response remained at the same level. The degraded antigen did not evoke the anaphylactic contraction of the terminal segment of the ileumin vitro, and moreover, it blocked the contraction after addition of the whole antigen.


Human Serum Albumin Anaphylactic Reaction Terminal Segment Intracellular Protease Passive Sensitization 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1974

Authors and Affiliations

  • J. Štefanovič
    • 1
    • 2
  • D. Kotulová
    • 1
    • 2
  • L’. Bergendi
    • 1
    • 2
  • I. Huzuláková
    • 1
    • 2
  1. 1.Department of Microbiology and Immunology, Faculty of MedicineComsnius UniversityBratislava
  2. 2.Department of Pharmacology, Faculty of MedicineComsnius UniversityBratislava

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