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Science in China Series B: Chemistry

, Volume 42, Issue 6, pp 617–623 | Cite as

Application of liquid pre-column capillary electrophoresis technique to the study of interaction between drug enantiomers and human serum albumin

  • Yongsheng Ding
  • Xiaofeng Zhu
  • Bingcheng Lin
Article

Abstract

Based on the chiral separation of several basic drugs, dimetindene, tetryzoline, theodrenaline and verapamil, the liquid pre-column capillary electrophoresis (LPC-CE) technique was established. It was used to determine free concentrations of dmg enantiomers in mixed solutions with human serum albumin (HSA). To prevent HSA entering the CE chiral srparation zone, the mobility differences between HSA and drugs under a specific pH condition were employed in thr LPC. Thus, the detection confusion caused by protein was totally avoided. Further study of binding constants determination and protein binding competitions was carried out. The study proves that the LPC technique could be used for complex mrdia, particularly the matrix of protein coexisting with a variety of dmgs.

Keywords

capillary electrophoresis liquid pre-column binding constant chiral drug human serum albumin 

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Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Yongsheng Ding
    • 1
  • Xiaofeng Zhu
    • 2
  • Bingcheng Lin
    • 2
  1. 1.Dalian Maritime UniversityDalianChina
  2. 2.Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina

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