Application of liquid pre-column capillary electrophoresis technique to the study of interaction between drug enantiomers and human serum albumin
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Based on the chiral separation of several basic drugs, dimetindene, tetryzoline, theodrenaline and verapamil, the liquid pre-column capillary electrophoresis (LPC-CE) technique was established. It was used to determine free concentrations of dmg enantiomers in mixed solutions with human serum albumin (HSA). To prevent HSA entering the CE chiral srparation zone, the mobility differences between HSA and drugs under a specific pH condition were employed in thr LPC. Thus, the detection confusion caused by protein was totally avoided. Further study of binding constants determination and protein binding competitions was carried out. The study proves that the LPC technique could be used for complex mrdia, particularly the matrix of protein coexisting with a variety of dmgs.
Keywordscapillary electrophoresis liquid pre-column binding constant chiral drug human serum albumin
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