Folia Microbiologica

, Volume 13, Issue 6, pp 510–514 | Cite as

Genetic transformation and transfection ofBacillus subtilis spheroplasts

  • P. Tichý
  • V. Rytíř
  • M. Kohoutová


Genetic transformation and transfection of lysozyme-treatedBacillus subtilis spheroplasts 168M ind occurs only if they are stabilized with 0.5m phosphate buffer and not if they are stabilized with 0.5m sucrose. Spheroplasts prepared from maximally competent cells give maximum transformation and transfection results. The results indicate that the DNA receptors must also be intact in the spheroplasts.


Bacillus Subtilis Indicator Culture Phage Titre Recipient Genome Plaque Count 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1968

Authors and Affiliations

  • P. Tichý
    • 1
  • V. Rytíř
    • 1
  • M. Kohoutová
    • 1
  1. 1.Department of Bacterial Genetics, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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