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Biosynthesis of methionine in an ethionine-resistant strain ofCandida utilis

Биосннтез метио нина у штамма Candida utilis, устойчивого к зтионину

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Abstract

The strainCandida utilis T 20 adapted to a high concentration of ethionine, excretes considerable amounts of methionine in a synthetic medium, about 40 times as much as the original non-adapted strain. At the same time, the amount of methionine in yeast cells incrncreased, predominantly in the pool (9 times as much as in the control). This ability to produce greater amounts of methionine in the pool or to excrete it into the medium is not permanent, since after 5 passages on agar with ut ethionine the amount of methionine was practically not increased as compared with the original non-adapted strain.

An increase in free methionine and of methionine excreted into the medium was found on cultivating the strain in a molasses-containing medium, too.

Abstract

Штамм Candida utilis T 20, адаптированный к высокой концетраации зтонина, выделяет в куль тивационную синтетическую среду значительное количество метионина,—приблизительно в 40 раз больще, чем первоначальный неадаптированный штамн. Одновременно повышается и содержание метионина в дрожжевых клетках, преимущественно в «pool» (в 9 раз больше, чем в контроле). Эта способность образовать большие количества метионина в «pool» или выделять его в среду оказывается непостоянной, так как после 5 пассажей на среде с агаром без этионина количество метионина практически не повышалось по сравнению с первоначальным неадаптированным штаммом.

Повышение содержания свободного метионина и метионина, выделяемого в среду, наблюдалось и при культивации адаптированного штамма на среде с мелассой.

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Authors and Affiliations

  1. Department of Technical Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

    Marie Musílková & Z. Fencl

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  1. Marie Musílková
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  2. Z. Fencl
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Musílková, M., Fencl, Z. Biosynthesis of methionine in an ethionine-resistant strain ofCandida utilis . Folia Microbiol 9, 374–379 (1964). https://doi.org/10.1007/BF02874189

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