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Folia Microbiologica

, Volume 9, Issue 3, pp 156–163 | Cite as

Relationship of sulphur metabolism and division ofChlorella pyrenoidosa cells

  • Dagmar Vraná
  • Z. Fencl
Article

Abstract

It was demonstrated that caproic acid inhibits the uptake of phosphate and sulphate byChlorella cells. These findings were used to demonstrate the existence of the previously hypothetical sulphur compound (Tamiyaet al. 1959–1961) essential to the process of division of the alga. A35S-labelled substance was isolated by partition chromatography on a DEAE cellulose column; it was found to be thermolabile and capable of inducing division of the algae even in an inhibited culture. It was also demonstrated that the addition of the substance during the early hours of ontogenetic development shortened the latter by almost half. It can be concluded that the dark phase in the terminal stage of ontogenetic development has a positive effect on the synthesis of this S-substance.

Keywords

DEAE Cellulose Caproic Acid Sulphur Metabolism Ontogenetic Development Developmental Cycle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Отношение между метаболизмом серы и клеточным делением у водоросли Chlorella pyrenoidosa

Abstract

В опытах было доказано, что капроновая кислота угнетает внедрение фосфата и сульфата в клетки водоросли Chlorella. Это наблюдение было использовано для обнаружения соединения серы, существование которого уже раньше предполагали Tamiya et al. (1959–1960) и которое необходимо в процессе деления водоросли. Вещество, меченое S35, было обнаружено с помощью хроматографии на колонке целлюлозы DEAE; было доказано, что выделенное термолабильние вещество способно вызывать деление водорослей и в угнетенной культуре. Далее, было доказано, что путем прибавления этого вещества в течение первых часов онтогенетического развития можно сократить это развитие почти наполовину. Можно полагать, что фаза темноты в конце онтогенетического развития оказывает положительное действие на синтез указанного S вещества.

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References

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1964

Authors and Affiliations

  • Dagmar Vraná
    • 1
  • Z. Fencl
    • 1
  1. 1.Department of Technical Microbiology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 6

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