Abstract
The influence of macronutrients and growth regulators on in vitro shoot proliferation and rooting of an East Spanish population ofMyrtus communis L. were studied. Preincubation of field material on a medium without mineral salts prevented the browning from phenolic exudates. For multiplication, nodal segments of 5 mm fromin vitro produced shoots were cultured on Murashige and Skoog (MS), Schenk and Hildebrandt (SH) and Heller (H) media (full strength or diluted to 1/2 or 1/4), with 6-benzylaminopurine (BAP) at concentrations 4.4, 13.3 and 22.2 ΜM or kinetin (K) at concentrations 4.7, 14.0 and 23.2 ΜM. The optimum shoot proliferation was on quarter-strength MS medium with 4.4 ΜM BAP, whereas the maximum number of nodal segments was produced on half-strength MS medium with 4.4 ΜM BAP. Rooting of shoots was obtained by adding 2.5 – 24.6 ΜM indole-3-butyric acid (IBA) and broad range of macronutrients; Lloyd and McCown (WPM) and Gresshoff and Doy (GD) media both full strength or diluted to 1/2 were optimum. No rooting was obtained in the presence of α-naphthaleneacetic acid (NAA).
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Abbreviations
- BAP:
-
6-benzylaminopurine
- GD:
-
Gresshoff and Doy (1972) medium
- H:
-
Heller (1953) mediwn
- IBA:
-
indole-3-butyric acid
- K:
-
kinetin
- MS:
-
Murashige and Skoog (1962) medium
- NAA:
-
α-naphthaleneacetic acid
- SH:
-
Schenk and Hildebrandt (1972) medium
- WPM:
-
LLoyd and McCown (1981) medium
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Acknowledgements: This study was supported by a grant from Conselleria de Cultura, Educació i Ciència de Ia Generalitat Valenciana. The authors are grateful to Man Cannen Perea for her helpful comments.
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Parra, R., Amo-Marco, J.B. Effect of plant growth regulators and basal media onin vitro shoot proliferation and rooting ofMyrtus communis L.. Biol Plant 38, 161–168 (1996). https://doi.org/10.1007/BF02873839
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DOI: https://doi.org/10.1007/BF02873839