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Biologia Plantarum

, 38:161 | Cite as

Effect of plant growth regulators and basal media onin vitro shoot proliferation and rooting ofMyrtus communis L.

  • R. Parra
  • J. B. Amo-Marco
Original Paper

Abstract

The influence of macronutrients and growth regulators on in vitro shoot proliferation and rooting of an East Spanish population ofMyrtus communis L. were studied. Preincubation of field material on a medium without mineral salts prevented the browning from phenolic exudates. For multiplication, nodal segments of 5 mm fromin vitro produced shoots were cultured on Murashige and Skoog (MS), Schenk and Hildebrandt (SH) and Heller (H) media (full strength or diluted to 1/2 or 1/4), with 6-benzylaminopurine (BAP) at concentrations 4.4, 13.3 and 22.2 ΜM or kinetin (K) at concentrations 4.7, 14.0 and 23.2 ΜM. The optimum shoot proliferation was on quarter-strength MS medium with 4.4 ΜM BAP, whereas the maximum number of nodal segments was produced on half-strength MS medium with 4.4 ΜM BAP. Rooting of shoots was obtained by adding 2.5 – 24.6 ΜM indole-3-butyric acid (IBA) and broad range of macronutrients; Lloyd and McCown (WPM) and Gresshoff and Doy (GD) media both full strength or diluted to 1/2 were optimum. No rooting was obtained in the presence of α-naphthaleneacetic acid (NAA).

Additional key words

auxins cytokinins macronutrients requirement micropropagation tissue culture 

Abbreviations

BAP

6-benzylaminopurine

GD

Gresshoff and Doy (1972) medium

H

Heller (1953) mediwn

IBA

indole-3-butyric acid

K

kinetin

MS

Murashige and Skoog (1962) medium

NAA

α-naphthaleneacetic acid

SH

Schenk and Hildebrandt (1972) medium

WPM

LLoyd and McCown (1981) medium

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Copyright information

© Institute of Experimental Botany, ASCR 1996

Authors and Affiliations

  • R. Parra
    • 1
  • J. B. Amo-Marco
    • 1
  1. 1.Departamento de Biologia Vegetal, Facultad de BiologiaUniversitat de ValenciaBurjassotSpain

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