Folia Microbiologica

, Volume 12, Issue 4, pp 345–349 | Cite as

The parasitism of the mouldPenicillium purpurogenum onAspergillus niger

I. Isolation of cell walls from the mycelium ofAspergillus niger grown by the surface method
  • J. Leopold
  • O. Seichertová


The mycelium from a surface culture of the fungusAspergillus niger was mechanically destroyed in a mixer. By this action three distinct particle types arose: isolated hyphae, hyphae agglomerates and granules of cytoplasm. The hyphae agglomerates were removed by fractional centrifugation, the hyphae were separated from the granules by repeated filtration over a sieve with openings 0.063 mm. The isolated hyphae were then deprived of the cytoplasm by the action of pancreatin. Thus obtained cell walls preserved the cell shape and were free of cell contents. The chemical composition of the walls exhibits a dispersion after the mycelium origin. The walls give a positive reaction on chitin, and contain 55 to 85% of the total carbohydrate contents (cellulose 2.5 to 5.5%), 1.5 to 2.1% of total N2 (0.8 to 0.9% of the amino-N2) and 8% of the lipides. After an acid hydrolysis, the presence of 45 to 59% of glucose, 9.5 to 14.5% of glucosamine, 1.5 to 6% of galactose, and small amounts of arabinose and mannose were determined. By qualitative chromatography, several aminoacids were ascertained.


Cell Wall Chitin Papain Pancreatin Mechanical Disintegration 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1967

Authors and Affiliations

  • J. Leopold
    • 1
  • O. Seichertová
    • 1
  1. 1.Biochemical Research of SpolanaKaznějov near Plzeň

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