Indian Journal of Clinical Biochemistry

, Volume 15, Issue 1, pp 17–21 | Cite as

Studies on the antiperoxide and antihaemolytic action of polyamines using human erythrocytes

  • Chethana Chetan
  • Ashok Prabhu
  • Vivian D'Souza
  • Shrinivas B. Rao
Free Radicals and Antioxidants


Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against the peroxidative action of H2O2 which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H2O2 and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines may have independent mechanism of action.

Key Words

Putrescine spermidine spermine lipid peroxidation erythrocytes haemolysis 


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Copyright information

© Association of Clinical Biochemists of India 2000

Authors and Affiliations

  • Chethana Chetan
    • 1
  • Ashok Prabhu
    • 1
  • Vivian D'Souza
    • 1
  • Shrinivas B. Rao
    • 1
  1. 1.Department of BiochemistryKasturba Medical CollegeMangalore

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