Folia Microbiologica

, Volume 15, Issue 3, pp 176–182 | Cite as

The method for estimation of opsonic activity in sera of infants

  • V. Jouja


Three methods for the estimation of opsonic activity in the sera of newborn children were tested. Two of them, based on the phagocytosis of opsonised bacteria labelled with radioactive phosphorus32P as measured byin vivo blood clearance or uptake of bacteria in perfused isolated liver, were found to be unsuitable for long term dynamic study mainly because they do not permit the testing of series of samples. The third method (using isolated phagocytic cellsin vitro) permits the differentiation of the opsonic effect of complement and antibody and, furthermore, the firmness of the bond between microbes and phagocytes (which reflects the degree of opsonization) can be established. It was found that a 2-mercaptoethanol-resistant antibody, probably of the IgG type, was responsible for the opsonic activity of children's sera toEscherichia coli 083. Homologous antibody (toEscherichia coli 083) could be differentiated from beterologous antibody (toEscherichia coli 086) using the opsonic test only at low dilutions of sera. The combination of newborn piglet complement and antibody of children's sera yielded higher values of opsonic activity than either component separately.


Ringer Solution Blood Clearance Phagocytic Index Liver Preparation Opsonic Activity 


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  1. Benacerraf, B., Sebestyen, M. M., Schlosman,S.:A quantitative study of the kinetics of blood clearance of P 32 labelled E. coli and staphylococci by the reticuloendothelial system. J. exptl. Med. 110: 27, 1959.CrossRefGoogle Scholar
  2. Biozzi, G., Benacerraf, B., Halpern, B. N.:II. A study of the kinetics of the granulopectic activity of the RES in relation to the dose of carbon injected. Relation between the weight of the organs and their activity. Brit. J. exptl. Pathol. 34: 441, 1958.Google Scholar
  3. Cooper, N. R., Fogel, B. J.:Complement in normal and disease processes. J. Pediat. 70: 982, 1967.PubMedCrossRefGoogle Scholar
  4. Fleischman, J. B., Pain, R. H., Porter, R. R.:Reduction of gamma-globulins. Arch. Biochem. Suppl. 1: 174, 1962.Google Scholar
  5. Hirsch, J. G., Strauss, B. J.:Studies on heat-labile opsonin in rabbit serum. Immunology 92: 145, 1964.Google Scholar
  6. Howard, J. G., Wardlaw, A. C.:The opsonic effect of normal serum on the uptake of bacteria by the reticuloendothelial system. Immunology. 1: 338, 1958.PubMedGoogle Scholar
  7. Lodinová, R.:Interaction of rabbit lymph node cells with bacteria (Salmonella paratyphi B). Fol. microbiol. 10: 275, 1965.CrossRefGoogle Scholar
  8. Lodinová, R. Jouja, V., Lanc, A.:Influence of the intestinal flora on the development of immune reactions in infants. J. Bacteriol. 93: 797, 1967.PubMedGoogle Scholar
  9. Neter, E. L. F., Bertram, D. A., Zak, M. R., Arbesman, C. E.:Studies on hemagglutination and hemolysis by Escherichia coli antisera. J. exptl. Med. 96: 1, 1952.CrossRefGoogle Scholar
  10. Smith, J. W., Barnert, J. A., May, R. P., Sanford, J. P.:Comparison of the opsonic activity of γ-G and γ-M antiproteus globulins. J. Immunol. 98: 336, 1967.PubMedGoogle Scholar
  11. Šterzl, J.:The opsonic activity of complement in sera without antibody. Fol. microbiol. 4: 240, 1963.CrossRefGoogle Scholar

Copyright information

© Academia, nakladatelství Československé akademie věd 1970

Authors and Affiliations

  • V. Jouja
    • 1
  1. 1.Institute for Care of Mother and ChildPrague-Podolí

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