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Indian Journal of Clinical Biochemistry

, Volume 12, Supplement 1, pp 68–71 | Cite as

Mycobacterium tuberculosis 38kDa antigen and its encoding gene-experience in diagnostic applications

  • G. V. Kadival
  • C. D. D’souza
  • M. Kameswaran
  • A. M. Samuel
Tuberculosis

Conclusion

Our experience has revealed that the detection of 38 kDa antigen or antibody to the antigen in various fluids is useful in diagnosis of various mainfestations of tuberculosis. The PCR developed for 340bp sequence of its encoding gene also shows a high degree of sensitivity and specificity. Thus the 38 kDa antigen/antibody combination or the PCR are ideal for development of kits for diagnosis. These immunoassays to be successful, isolation of the 38 kDa antigen in large quantities is essential. Using recombinant DNA technology and expression inE. coli this has been achieved. However, such recombinant antigen does not have the same immunological properties as the native antigen and hence not suitable in immunodiagnosis. To fully realise the potential of the MoAb defined antigens such as the 38 kDa antigen, 19 kDa antigen and others it is essential to devise alternative vector-host systems that help in glycosylation, do not accumulate as inclusion bodies and can be isolated with less damage.

Key Words

38kDa antigen Over expression PCR Dot-ELISA 

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References

  1. 1.
    Bloom, B.R. and Murray, C.J.L. (1992) Tuberculosis-Commentary on a reemergent killer, Science, 257 1055–1064.PubMedCrossRefGoogle Scholar
  2. 2.
    Kadival, G.V. and Chaparas, S.D. (1987) Production, characterisation and species specificity of five monoclonal antibodies toMycobacterium tuberculosis. J.Clin. Microbiol. 25, 76–80.PubMedGoogle Scholar
  3. 3.
    Kadival, G.V., Chaparas, S.D. and Hussong D. (1987) Characterisation of serologic and cell-mediated reactivity of a 38 kDa antigen isolated fromMycobacterium tuberculosis. J. Immunol. 139, 2447–2451.PubMedGoogle Scholar
  4. 4.
    Kadival, G.V., Kameswaran, M., Ashtekar, M.D. and Samuel, A.M. (1990) immunodiagnosis of tuberculosis using polyclonal and monoclonal antibodies Trop. Med. Parasitol. Suppl. 41, 363.Google Scholar
  5. 5.
    Kameswaran, M., Kadival, G.V., Samuel, A.M., Kale, R. and Virdi, S.S., (1989) A simple dot blot ELISA for antigen ofM. tuberculosis. Indian J. Tuber. 36 103.Google Scholar
  6. 6.
    Kadival, G.V., Kameswaran, M., Joshi, R., Todiwala, S.S. and Samuel, A.M. (1994). Detection of antibodies to a defined antigen (38kDa) ofM. tuberculosis in CSF patients with CNS disorder. Zentralblatt fur Mikrobiologie 281, 95.Google Scholar
  7. 7.
    Kadival, G.V., D'Souza, C.D., Kolk, A.H.J. and Samuel, A.M. (1990) Diagnosis of tuberculosis by Polymerase chain reaction comparison of two targets for amplification. Zentralblatt fur Mikrobiologie 282, 353.Google Scholar
  8. 8.
    Kadival, G.V., D'Souza, C.D., Kulkarni, S.P. and Samuel A.M. (1996) A Highly Specific Polymerase Chain Reaction Test for the Detection ofM. tuberculosis. Indian J. Tuberculosis 43, 151.Google Scholar
  9. 9.
    Singh, M., Andersen, A.B., McCarthy, J.E.G., Rhode, M., Schutte, H., Sanders, E. and Timmis, K.N. (1992) The Mycobacterium tuberculosis 38kDa antigen: overproduction inEscherichia coli, purification and characterisation. Gene, 117, 53–60.PubMedCrossRefGoogle Scholar
  10. 10.
    D'Souza, C.D., Kadival, G.V. and Samuel, A.M (1994) Use of the recombinant 38kDa antigen ofM. tuberculosis as an immunogen for specific antisera production. Microbiology and Immunology 38: 797.PubMedGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 1997

Authors and Affiliations

  • G. V. Kadival
    • 1
  • C. D. D’souza
    • 1
  • M. Kameswaran
    • 1
  • A. M. Samuel
    • 1
  1. 1.Radiation Medicine Centre, Bhabha Atomic Research CentreTata Memorial Centre AnnexeMumbai

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