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Folia Microbiologica

, Volume 29, Issue 2, pp 148–155 | Cite as

Mineralization of 2,4-dichlorophenoxyacetic acid in soil simultaneously enriched with saccharides

  • F. Kunc
  • J. Rybářová
  • J. Lasík
Article

Abstract

Detoxication of 2,4-dichlorophenoxyacetic acid (2,4-D) in samples of chernozem soil was determined by a biological test and the time course of production of14CO2 a product of microbial degradation of 2-14C-2,4-D, was measured during 38-d incubation at 28°C in the dark. Enrichment of the soil with glucose (1000 ppm), two exocellular bacterial glucan and glucomannan polysaccharides (750 ppm), or a mixture of glucose with (NH4)2SO4 (C:N=5∶1) brought about acceleration of both detoxication and mineralization of 2,4-D (50 ppm) added simultaneously with the saccharides. Mineralization of the saccharides always preceded the degradation of the herbicide. The lag phase of 2,4-D mineralization, did not exceed 3 d. In samples with saccharides the doubling time of the mineralization activity in the exponential phase of the process was substantially shortened and the mineralization of 2,4-D was accelerated even when the soil was inoculated with a suspension of soil in which microbial 2,4-D decomposers had accumulated. The extent, of mineralization was not affected by the presence of saccharides (about 1/3 of the introduced radioactive carbon was transformed into14CO2). All saccharides had a similar effect which reflected an increase in the overall bacterial count and in the relative abundance of bacterial 2,4-D decomposers. The role of other mechanisms such as co-metabolism in the stimulation of the degradation process is discussed.

Keywords

MCPA Picloram Dicamba Chernozem Soil Microbial Degradation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1984

Authors and Affiliations

  • F. Kunc
    • 1
  • J. Rybářová
    • 1
  • J. Lasík
    • 1
  1. 1.Department of Microbial Ecology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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