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Function of terminal acceptors in the biosynthesis of denitrification pathway components inParacoccus denitrificans

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Abstract

Limited aeration of cell suspension in growth medium was used to study the kinetics of formation of nitrite reductase and nitrous-oxide reductase and their physiological electron donor, cytochromec-550, during the anaerobic adaptation ofParacoccus denitrificans. The crucial step in the regulation of synthesis of these components is the repressive effect of oxygen while nitrogenous acceptors (NO3 −, NO2 −, N2O) probably play no role as inducers. The time course of the enzyme activites was analogous (after a lag phase a sharp increase with a maximum after 3 h) and differed from the kinetics of synthesis of cytochromec-550 (gradual rise throughout the 8-h experiment).

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Sciences, J.E. Purkyně University, 611 37, Brno, Czechoslovakia

    I. Kučera, P. Boublíková & V. Dadák

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  1. I. Kučera
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  2. P. Boublíková
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  3. V. Dadák
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Kučera, I., Boublíková, P. & Dadák, V. Function of terminal acceptors in the biosynthesis of denitrification pathway components inParacoccus denitrificans . Folia Microbiol 29, 108–114 (1984). https://doi.org/10.1007/BF02872925

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  • DOI: https://doi.org/10.1007/BF02872925

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