Function of terminal acceptors in the biosynthesis of denitrification pathway components inParacoccus denitrificans
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Limited aeration of cell suspension in growth medium was used to study the kinetics of formation of nitrite reductase and nitrous-oxide reductase and their physiological electron donor, cytochromec-550, during the anaerobic adaptation ofParacoccus denitrificans. The crucial step in the regulation of synthesis of these components is the repressive effect of oxygen while nitrogenous acceptors (NO3 −, NO2 −, N2O) probably play no role as inducers. The time course of the enzyme activites was analogous (after a lag phase a sharp increase with a maximum after 3 h) and differed from the kinetics of synthesis of cytochromec-550 (gradual rise throughout the 8-h experiment).
KeywordsNitrite Nitrous Oxide Nitrate Reductase Nitrite Reductase Paracoccus Denitrificans
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