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Folia Microbiologica

, Volume 19, Issue 5, pp 373–377 | Cite as

Studies on methanol-oxidizing yeast

II. Lipids
  • O. Volfová
  • J. Panoš
  • K. Pecka
Article

Abstract

The yeast strain 11Bh was studied from the aspect of qualitative and qunatitative composition of lipids formed in cells during growth on methanol, synthetic ethanol and glucose. The strain was found to form some 3% free fatty acids toward the end of the growth phase. More esterified fatty acids are formed on ethanol and glucose (2.75 and 2.86%, respectively) than on methanol (1.6%). The composition of lipids and representation of the various fatty acids in the lipids is similar on all three substrates. The cell lipids contain over 40 rel.% oleic and about 16 rel.% each of palmitoleic, palmitio and linolenic acid. Odd-numbered fatty acids are present after growth on any of the three substrates, amounting to at most 4 rel.%. Of the extracellular fatty acids formed toward the end of growth of cells on methanol, propionic and acetic acid occurred in largest amounts in the medium.

Keywords

Free Fatty Acid Triolein Dissolve Oxygen Tension Candida Utilis Molybdic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bligh E. G., Dyer W. J.: A rapid method of total lipid extraction and purification.Can. J. Biochem. Physiol. 37, 911 (1959).PubMedGoogle Scholar
  2. Brown C. M., Rose A. H.: Fatty-acid composition ofCandida utilis as affected by growth temperature and dissolved oxygen tension.J. Bacteriol. 99, 371 (1969).PubMedGoogle Scholar
  3. Cooney C. L., Levine D.: Microbial utilization of methanol.Adv. Appl. Microbiol. 15, 337 (1972).PubMedGoogle Scholar
  4. Dietz W. A.: Response factors for gas chromatographic analyses.J. Gas Chromat. 5, 68 (1967).Google Scholar
  5. Dyatlovitskaya E. V., Greshnykh K. P., Bergel'son L. D.: Chemistry of lipids. IV. Lipids of yeast grown on normal alkanes. (In Russian)Prikl. Biokhim. Mikrobiol. 1, 613 (1965).Google Scholar
  6. Kates M., Baxter R. M.: Lipid composition of mesophilic and psychrophilic yeasts (Candida sp.) as influenced by environmental temperature.Can. J. Biochem. Physiol. 40, 1213 (1962).PubMedGoogle Scholar
  7. Lideman L. S., Vortilo C. P., Finkelshtein Z. I.: Synthesis of fatty acids by paraffin-oxidizing yeastCandida lipolytica No. 670. (In Russian)Mikrobiol. Sintez 8, 21 (1969).Google Scholar
  8. Melcalfe L. D., Schmitz A. A., Pelka J. R.: Rapid preparation of fatty acids ester from lipids for gas chromatographic analysis.Anal. Chem. 38, 514 (1966).CrossRefGoogle Scholar
  9. Mizuno M., Shimosima, Y., Iguchi, T., Takeda I., Senoh S.: Fatty acid composition of hydrocarbonassimilating yeast.Agr. Biol. Chem. 30, 506 (1966).Google Scholar
  10. Novák M.: Colorimetric ultramicro method for the determination of free fatty acids.J. Lipid Res. 6 431 (1965).PubMedGoogle Scholar
  11. Ogata K., Nishikawa H., Ohsugi M.: A yeast capable of utilizing methanol.Agr. Biol. Chem. 33, 1519 (1969).Google Scholar
  12. Ogata K., Nishikawa H., Ohsugi M., Tochikura T.: Studies on the production of yeast. II. The cultural conditions of methanol assimilating yeast.Kloeckera sp. No. 2201.J. Ferment. Technol. 48, 470 (1970).Google Scholar
  13. Quayle J. R.: The metabolism of one-carbon compounds by microorganisms.Adv. Microbiol. Physiol. 7, 119 (1972).Google Scholar
  14. Sahm H., Wagner F.: Mikrobielle Verwertung von Methanol. Isolierung und Charakterisierung der HefeCandida boidinii.Arch. Mikrobiol. 84, 29 (1972).PubMedCrossRefGoogle Scholar
  15. Volfová O., Pecka K.: Cultivation ofCandida lipolytica 4-1 on hydrocarbons. IV. Fatty acids formed during batch cultivation on model gas oils.Folia Microbiol. 18, 286 (1973).CrossRefGoogle Scholar
  16. Volfová O., Pilát P.: Studies on methanol-oxidizing yeast. I. Isolation and growth studies.Folia Microbiol. 19, 249 (1974).Google Scholar
  17. Wenke M., Wenke I., Šíp A.: The serial determination of lipids in organs using a special homogenizer.Čs. Fysiol. 6 251 (1957).Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1974

Authors and Affiliations

  • O. Volfová
    • 1
    • 2
  • J. Panoš
    • 1
    • 2
  • K. Pecka
    • 1
    • 2
  1. 1.Department of Technical Microbiology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4
  2. 2.College of Chemical TechnologyPrague 6

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