The Botanical Review

, Volume 22, Issue 10, pp 665–695 | Cite as

Fixation of plant chromosomes

Principles, limitations, recent developments
  • Arun Kumar Sharma


Colchicine Botanical Review Chromosome Structure Chromic Acid Somatic Chromosome 
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  1. 1.
    Backman, E. 1935. A rapid combined fixing and staining method for plant chromosome counts. Stain Tech.10: 83–86.Google Scholar
  2. 2.
    Baker, J. R. 1933. Cytological technique. Methuen & Co. Ltd.Google Scholar
  3. 3.
    Barber, H. N. 1940. The suppression of meiosis and the origin of diplo-chromosomes. Proc. Roy. Soc. B.128: 170–185.Google Scholar
  4. 4.
    —— andH. G. Callan. 1943. The effects of cold and colchicine on mitosis in the newt. Proc. Roy. Soc. B.131: 258–271.Google Scholar
  5. 5.
    Beams, H. W., andR. L. King. 1938. An experimental study on mitosis in the somatic cells of wheat. Biol. Bull.75: 189–207.CrossRefGoogle Scholar
  6. 6.
    Belling, J. 1926. The iron aceto-carmine method of fixing and staining chromosomes. Biol. Bull.50: 160–162.CrossRefGoogle Scholar
  7. 7.
    ——. 1928. A method for the study of chromosomes in pollen mother-cells. Univ. Calif., Pub. Bot.14: 293–299.Google Scholar
  8. 8.
    Benda, C. 1902. Ergeb. Anat. Entw.12: 743. [Referred to Baker’s “Cytological technique”].Google Scholar
  9. 9.
    Bhaduri, P. N. 1939. A study of the effects of different forms of colchicine on roots ofVicia faba L. Jour. Roy. Micr. Soc.59: 245–276.Google Scholar
  10. 10.
    C. S. Semmens. 1940. Advantages of uranium fixation in modern cytological technique. Nature [London]146: 100.Google Scholar
  11. 11.
    —— andA. K. Sharma. 1946. Cytogenetics ofDatura fastuosa L. Bull. Torrey Bot. Club73: 438–450.CrossRefGoogle Scholar
  12. 12.
    Bowden, W. M. 1949. Some modifications of Warmke’s permanent section smear method for plant chromosomes. Stain Tech.24: 71–76.Google Scholar
  13. 13.
    Brachet, J. 1940. La détection histochémique des acides pentosenucléiques. Comp. Rend. Soc. Biol.133: 88–90.Google Scholar
  14. 14.
    Burch, C. R., andJ. P. J. Stock. 1942. Phase contrast microscopy. Jour. Sci. Inst.19: 71.CrossRefGoogle Scholar
  15. 15.
    Burrell, P. C. 1939. Root-tip smear method for difficult material. Stain Tech.14: 147–149.Google Scholar
  16. 16.
    Callan, H. G. 1942. Heterochromatin inTriton. Proc. Roy. Soc. B.130: 324–335.Google Scholar
  17. 17.*
    Carnoy, J. B. 1886. La Cellule3: 1, 227. [Referred to in Baker’s “Cytological technique”].Google Scholar
  18. 18.
    Carey, M. A., andE. S. McDonough. 1943. On the production of polyploidy inAllium with paradichlorobenzene. Jour. Hered.34: 238–240.Google Scholar
  19. 19.*
    Champy, C. 1913. Arch. Zool. Exper. [Referred to in Bronte-Gatenby and T. S. Painter, “Microtomist’s Vade Mecum”].Google Scholar
  20. 20.
    Cohen, I. 1949. Sudan Black B—a new stain for chromosome smear preparation. Stain Tech.24: 177–184.Google Scholar
  21. 21.
    Cole, E. C. 1926. A rapid iron haematoxylin technique. Science64: 452–453.PubMedCrossRefGoogle Scholar
  22. 22.
    Conagin, andCandida, H. T. 1951. Paradichlorobenzene for chromosome counts. Stain Tech.26: 274.Google Scholar
  23. 23.
    Darlington, C. D., andL. La Cour. 1940. Nucleic acid starvation in chromosomes ofTrillium. Jour. Genet.40: 185–213.Google Scholar
  24. 24.
    Dermen, Haig, andD. H. Scott. 1950. Chromosome counts in apple and strawberry aided by paradichlorobenzene. Proc. Amer. Soc. Hort. Sci.56: 145–148.Google Scholar
  25. 25.
    Di Stefano, H. S. 1952. Feulgen hydrolysis with hydrochloric acid. Stain Tech.27: 171–174.Google Scholar
  26. 26.
    Emsweller, S. L., andN. W. Stuart. 1944. Imphoving smear technic by the use of enzymes. Stain Tech.19: 109–114.Google Scholar
  27. 27.
    Faberge, A. C. 1945. Snail stomach cytase—a new reagent for plant cytology. Stain Tech.20: 1–4.Google Scholar
  28. 28.
    Ferguson, J. 1939. The use of chemical potentials as indices of toxicity. Proc. Roy. Soc. B.127: 387–404.CrossRefGoogle Scholar
  29. 29.
    Feulgen, R., andH. Rossenbeck. 1924. Microscopisch-chemisches Nachweis einer Necleinsaure vom Typus der Thymonucleinsaure. Zeits. Physiol. Chemie135: 203–248.Google Scholar
  30. 30.
    Flemming, W. 1882. Zellsubstanz Kern und Zelltheilung. [Referred to in Baker’s “Cytological Technique”].Google Scholar
  31. 31.*
    ——. 1884. Zeit. Wiss. Mik.1: 349. [Referred to in Baker’s “Cytological Technique”].Google Scholar
  32. 32.
    Gavauden, P., M. Dode andH. Poussel. 1945. L’importance de la notion d’activité thermodynamique en toxicologie et en pharmacodynamie. Rec. Trav. Sci. Stat. d’Ess Bouchet1: 5–22.Google Scholar
  33. 33.
    Gersh, I. 1932. The Altmann technique for fixation by drying while freezing. Anat. Rec.53: 309.CrossRefGoogle Scholar
  34. 34.
    ——. 1948. Application in pathology of the method of fixation by freezing and drying of tissues. Bull. Int. Assoc. Med. Mus.28: 179.Google Scholar
  35. 35.
    Gerstell, D. U. 1949. Hydrochloric acid as fixative for root-tip chromosomes. Stain Tech.24: 95–97.Google Scholar
  36. 36.
    Hanson, A. A., andD. A. Oldemeyer. 1951. Staining root-tip smears with aceto-carmine. Stain Tech.26: 241–242.Google Scholar
  37. 37.
    Hillary, B. B. 1939. Improvements in the permanent root-tip squash technic. Stain Tech.14: 97–99.Google Scholar
  38. 38.
    Johnson, L. P. V. 1945. A rapid squash technic for stem and root-tips. Canad. Jour. Res. C.23: 129–130.Google Scholar
  39. 39.
    Koller, P. C. 1947. The integration of nuclear and cytoplasmic activities in the cell. Proc. VI Int. Cong. Exp. Cytol: 85–92.Google Scholar
  40. 40.
    Kostoff, D. 1938. Irregularities in the mitosis and polyploidy induced by colchicine and acenaphthene. Comp. Rend. (Doklady) Acad. Sci. U. S. S. R.19: 197–199.Google Scholar
  41. 41.
    Kurnick, J. B. 1950. Methyl green pyronin.—I. Basis of selective staining of nuclei acids. Jour. Gen. Phqsiol.33: 243–264.CrossRefGoogle Scholar
  42. 42.
    Kuwada, Y., N. Shinke andG. Oura. 1938. Artificial uncoiling of the chromonema spirals as a method of investigation of the chromosome structure. Zeits. Wiss. Mikr.55: 8–16.Google Scholar
  43. 43.
    La Cour, L. F. 1931. Improvements in everyday technique in plant cytology. Jour. Roy. Micr. Soc.51: 119–126.Google Scholar
  44. 44.
    ——. 1936. Technic for studying chromosome structure. Stain Tech.10: 57–59.Google Scholar
  45. 45.
    ——. 1937. Improvements in plant cytological technique. Bot. Rev.5: 241–258.CrossRefGoogle Scholar
  46. 46.
    ——. 1941. Acetic orcein. Stain Tech.16: 169–174.Google Scholar
  47. 47.
    ——. 1947. Improvements in plant cytological technique. II. Bot. Rev.15: 216–240.CrossRefGoogle Scholar
  48. 48.
    —— andA. C. Faberge. 1943. The use of cellophane in pollen tube technic. Stain Tech.18: 196.Google Scholar
  49. 49.
    Law, A. G. 1943. Root-tip smears following fixation with boiling water. Stain Tech.18: 117–118.Google Scholar
  50. 50.
    Levan, A. 1938. The effect of colchicine on root mitoses inAllium. Hereditas24: 471–486.Google Scholar
  51. 51.
    --. 1949. Influence of chemicals on chromosomes as studied by theAllium test. Hereditas, Suppl. Vol.: 326–337.Google Scholar
  52. 52.
    —— andG. Ostergren. 1943. The mechanism of C-mitotic action. Hereditas29: 381–443.Google Scholar
  53. 53.
    Lewitsky, G. A. 1931. An assay on cytological analysis of the fixing action of the chrom-acetic-formalin and chromic-formalin. Bull. Appl. Bot.27: 76–85.Google Scholar
  54. 54.
    ——. 1931. The morphology of chromosomes. Bull. Appl. Bot.27: 19–174.Google Scholar
  55. 55.
    ——. 1934(a). New fixing mixtures revealing the morphology of the chromosomes. Comp. Rend. Acad. Sci. URSS.4: 144–145.Google Scholar
  56. 56.
    ——. 1934(b). Fixation changes of the chromosome body. Comp. Rend. Sci. URSS4: 223–224.Google Scholar
  57. 57.
    Ludford, R. J. 1936. The action of toxic substances upon the division of normal and malignant cells in vitro andin vivo. Arch. Exp. Zellf.18: 411–441.Google Scholar
  58. 58.
    Marks, G. E. 1952. A controllable carmine technique for plants with small chromosomes. Stain Tech.27: 333–336.Google Scholar
  59. 59.
    Matsuura, H. 1937. Chromosome studies onTrillium kamtschaticum Pall. V. Cytologia, Fujii Jub. Vol.: 20–34.Google Scholar
  60. 60.
    McClintock, B. 1929. A method for making aceto-carmine smears permanent. Stain Tech.4: 53–56.Google Scholar
  61. 61.
    McKay, H. H., andA. E. Clarke. 1946. The use of enzymes in the preparation of root-tip smears. Stain Tech.21: 111–114.Google Scholar
  62. 62.
    Mensinkai, S. W. 1939. The structure and behaviour of chromosomes in somatic mitosis. Jour. Roy. Micr. Soc.59: 82–112.Google Scholar
  63. 63.
    Meyer, J. R. 1943. Colchicine-Feulgen leaf smears. Stain Tech.18: 535–536.Google Scholar
  64. 64.
    ——. 1945. Prefixing with paradichlorobenzene to facilitate chromosome study. Stain Tech.20: 121–125.Google Scholar
  65. 65.
    Michel, K. 1941. Die Darstellung von Chromosomen mittels des Phasencontrastverfahrens. Naturwiss.29: 570–597.CrossRefGoogle Scholar
  66. 66.
    Mittler, S., andA. S. Bartha. 1948. Brilliant cresyl blue as a stain for chromosome smear preparations. Stain Tech.23: 27–29.Google Scholar
  67. 67.
    Navaschin, M. 1934. Die Methodik der zytologischen Untersuchungen fur zuchterische Zwecke. Zeits. Zücht. A,19: 366–413.Google Scholar
  68. 68.
    Newcomer, E. H. 1953. A new cytological and histological fixing fluid. Science118: 161.PubMedCrossRefGoogle Scholar
  69. 69.
    Newton, W. C. F. 1927. Chromosome studies inTulipa and some related genera. Jour. Linn. Soc., Bot.47: 336–354.Google Scholar
  70. 70.
    Oehlkers, F. 1952. Chromosome breaks influenced by chemicals. Heredity, Suppl. vol.6: 95–105.Google Scholar
  71. 71.
    O’Mara, J. G. 1939. Observations on the immediate effects of colchicine. Jour. Hered.30: 35–37.Google Scholar
  72. 72.
    ——. 1948. Acetic acid methods for chromosome studies at prophase and metaphase in meristems. Stain Tech.23: 201–204.Google Scholar
  73. 73.
    Ostergren, G., andA. Levan. 1943. The connection between C-mitotic activity and water solubility in some monocyclic compounds. Hereditas29: 496–498.Google Scholar
  74. 74.
    Peto, H. H. 1935. Association of somatic chromosomes induced by heat and chloral hydrate treatments. Canad. Jour. Res. C.13: 301–314.Google Scholar
  75. 75.
    Remak, R. 1854. Arch. Anat. Path. Wiss. Med. p. 99. [Referred to in Baker’s “Cytological Technique”].Google Scholar
  76. 76.
    Richards, O. W. 1944. Phase difference microscopy for living unstained protoplasm. Anat. Rec.89: 548.Google Scholar
  77. 77.
    Satina, S., A. D. Bergner, andA. F. Blakeslee. 1941. Morphological differentiation in chromosomes ofDatura stramonium. Am. Jour. Bot.28: 383–390.CrossRefGoogle Scholar
  78. 78.
    Schmuk, A. 1938. The chemical nature of substances inducing polyploidy in plants. Comp. Rend. (Doklady) Acad. Sci. U.S.S.R.19: 189–192.Google Scholar
  79. 79.
    Schreiber, J. 1951. A schedule for the study of somatic chromosomes inBromus. Stain Tech.26: 247–250.Google Scholar
  80. 80.
    Sharma, A. K. 1951. Trichloroacetic acid and Feulgen staining. Nature167: 441–442.PubMedCrossRefGoogle Scholar
  81. 81.
    ——. 1952. Desoxyribonucleic acid—The problem of its function, location and reactions. Port. Acta Biol.3: 289–317.Google Scholar
  82. 82.
    —— andChitra Ghosh. 1950. Oxyquinoline as an ingredient in a new fixative for chromosome analysis. Sci. Cult.16: 268–269.Google Scholar
  83. 83.
    —— and ——. 1951. Oxyquinoline—its possibilities in other cytological procedures. Sci. Cult.16: 528–529.Google Scholar
  84. 84.
    —— andDipti Shattacharjee. 1952. Permanent mounts of chromosomes after 8-oxyquinoline and—squashing. Stain Tech.27: 201–203.Google Scholar
  85. 85.
    —— andA. K. Bal. 1953. Coumarin in chromosome analysis. Stain Tech.28: 255–257.Google Scholar
  86. 86.
    -- and --. 1954. A cytological study of a few genera of Amaryllidaceae with a view to find out the basis of their phylogeny. Proc. 41st Indian Sci. Congr. [Abst.] 148.Google Scholar
  87. 87.
    —— andS. Sen. 1954. Study of effect of water and nuclear constituents. Genet. Iber.6: 19–32.Google Scholar
  88. 88.
    —— andN. K. Bhattacharyya. 1954. Treatment of root-tips in phenol for the study of karyotype. Curr. Sci.23: 232–233.Google Scholar
  89. 89.
    —— and (Miss)Archana Mookerjea. 1954. Possibilities of use of hormones in chromosome analysis. Caryologia6: 52–62.Google Scholar
  90. 90.
    —— and ——. 1955. Paradichlorobenzene and other chemicals in chromosome work. Stain Tech.30: 1–7.Google Scholar
  91. 91.
    -- andS. Sarkar. 1955(a). Veratrine—its use in cytochemistry. Proc. 42nd Indian Sci. Congr. [Abst.] 251–252.Google Scholar
  92. 92.
    —— and ——. 1955(b). A new technique for the study of the chromosomes of palms. Nature176: 261.PubMedCrossRefGoogle Scholar
  93. 93.
    —— andDeepesh De. 1955. Gallic acid—its importance in cytochemical studies. Caryologia6: (2–3): 180–189.Google Scholar
  94. 94.
    —— and (Miss)Mira Roy. 1955. Orcein staining and the study of the effect of chemicals on chromosomes. Chromosoma7: 275–280.PubMedCrossRefGoogle Scholar
  95. 94a.
    -- andBibha Bhattacharyya. 1955. Vitamins—their property of inducing division in permanent cells. Proc. 42nd Indian Sci. Congr. [Abst.] 251.Google Scholar
  96. 94b.
    —— andA. Datta. 1955. Ascorbic acid—its role in inducing division in permanent cells. Proc. 42nd Indian Sci. Congr. [Abst.] 249 and ΦΨTON6: 71–78 (1956).Google Scholar
  97. 95.
    Simpson, W. L. 1941. An experimental analysis of the Altmann technic of freezing drying. Anat. Rec.80: 173.CrossRefGoogle Scholar
  98. 96.
    Steinegger, E., andA. Levan. 1947. Constitution and C-mitotic activity of isocolchicine. Hereditas, Suppl.33: 385–396.CrossRefGoogle Scholar
  99. 97.
    Stewart, W. N., andA. M. Schertiger. 1949. Brilliant cresyl blue as a stain for plant chromosomes. Stain Tech.24: 39–45.Google Scholar
  100. 98.
    Stowell, R. E. 1951. A modified freezing drying apparatus for tissues. Stain Tech.26: 105–108.Google Scholar
  101. 99.
    Swanson, C. P. 1940. The use of acenaphthene in pollen tube technic. Stain Tech.15: 49–52.Google Scholar
  102. 100.
    Telleyniczky, K. 1898, 1902, 1905. Arch. Mikr. Anat.52, 60, 66 [Referred to in Baker’s “Cytological Technique”].Google Scholar
  103. 101.
    Tjio, J. H., andA. Levan. 1950. The use of oxyquinoline in chromosome analysis. Anal. Estac. Exp. Aula Dei2: 21–64.Google Scholar
  104. 102.
    —— and ——. 1950. Quadruple structure of the centromere. Nature165: 368.CrossRefGoogle Scholar
  105. 103.
    Warmke, H. E. 1935. A permanent root-tip smear method. Stain Tech.10: 101–103.Google Scholar
  106. 104.
    ——. 1941. A section smear method for plant cytology. Stain Tech.76: 9–12.Google Scholar
  107. 105.
    ——. 1946. Pre-cooling combined with chrom-osmo-acetic fixation in studies of somatic chromosomes in plants. Stain Tech.21: 87–89.Google Scholar
  108. 106.
    Whittaker, T. W., andG. W. Bohn. 1950. A smear technic for the Cucurbitaceae. Stain Tech.25: 133–136.Google Scholar
  109. 107.
    Zirkle, C. 1940. Combined fixing, staining and mounting media. Stain Tech.15: 139–153.Google Scholar

Copyright information

© The New York Botanical Garden 1956

Authors and Affiliations

  • Arun Kumar Sharma
    • 1
  1. 1.Cytogenetics Laboratory, Botany Dept.Calcutta UniversityCalcutta 19India

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