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Folia Microbiologica

, Volume 17, Issue 1, pp 17–27 | Cite as

Esterases in mycobacteria

I. Carboxylic ester hydrolases in a submerged culture ofMycobacterium phlei
  • P. Procházka
  • M. Nohýnek
  • A. Šroglová
  • J. Rokos
Article

Abstract

It was found that a submerged culture ofMycobacterium phlei degrades simple esters (ethylacetate and ethylbutyrate) as well as synthetic lipids (triacetine and tributyrine). The effect of pH on the rate of degradation of tributyrine was investigated and the maximum activity of esterases found within a wide range of pH. The activity of esterases was followed during growth of a submerged culture ofMycobacterium phlei. Esterases were not released into the cultivation medium during growth or even during the early stationary phase. Only a low steady activity of esterases could be demonstrated in a filtrate of the cultivation liquid. The total activity of esterases reached its maximum after a 6–11 day incubation. The specific activity of esterases reached a maximum on the 6th day of incubation; its value decreased to about one half and did not change substantially on prolonged incubation. Changes in the specific activity of esterases were found to be time-related with changes of pH and a decrease of the specific activity was associated with a release of macromolecular compounds into the incubation medium. Esterases as well as other macromolecular compounds were isolated from the filtrate of the cultivation medium ofMycobacterium phlei. The isolated preparation contained 60–72% total activity of esterases present in the filtrate of the cultivation liquid.

Keywords

Lipase Culture Filtrate Esterase Activity Submerged Culture Myco 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Academia, Publishing House of the Czechoslovak Academy of Sciences 1972

Authors and Affiliations

  • P. Procházka
    • 1
  • M. Nohýnek
    • 1
  • A. Šroglová
    • 1
  • J. Rokos
    • 1
  1. 1.Department of General Microbiology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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