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Xenotransplantation in immunosuppressed nude mice of human solid tumors and acute leukemias directly from patients orin vitro cell lines

  • Patrizia Caretto
  • Marco Forni
  • Gabriella d’Orazi
  • Susanna Scarpa
  • Paola Feraiorni
  • Cristina Jemma
  • Andrea Modesti
  • Manlio Ferrarini
  • Silvio Roncella
  • Robin Foà
  • Guido Forni
Original Contributions

Summary

Athymic nude (nu/nu) mice are widely employed for the heterotransplantation of human tumor cell lines establishedin vitro and tumor cells directly grafted from patients. By contrast, hemopoietic malignancies have consistently proved difficult to transplant and well-characterized human leukemias suitable for studies in nude mice are scarce. We report here our experience with subcutaneous xenotransplantation of human neoplastic cells into nu/nu mice immunosuppressed through sublethal irradiation and splenectomy (SI-nu/nu) and with an additional injection of anti-Asialo-GM1 antibodies (SIA-nu/nu) in order to eliminate natural killer activity. Thirteen out of 16 continuous cell lines establishedin vitro from solid tumors and 7 out of 14 human tumors obtained from fragments of surgical specimens formed a progressively growing tumor in SI-nu/nu mice. Six out of 8in vitro established human leukemic cell lines and 5 out of 18 neoplastic hematopoietic cells directly xenotransplanted from the patient grew SIA-nu/nu mice. When the membrane and chromosome markers of neoplastic cells that grew into the mice were evaluated, only marginal differences with those of the original tumors were found. In addition, when interfering factors alter the histological aspect of the primary tumor, xenotransplantation may also be of some help in histological diagnosis. By using SI- and SIA-nu/nu mice, it is thus possible to build up several newin vivo experimental systems with fresh human tumors that may be of value in studying the efficacy of differentiation factors and immunological maneuvers on thein vivo growth of human tumors.

Key-words

Human leukemias Human tumors Immunosuppressed nude mice Nude mice Xenotransplantation 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Patrizia Caretto
    • 1
  • Marco Forni
    • 3
  • Gabriella d’Orazi
    • 4
  • Susanna Scarpa
    • 4
  • Paola Feraiorni
    • 1
  • Cristina Jemma
    • 1
  • Andrea Modesti
    • 4
  • Manlio Ferrarini
    • 5
  • Silvio Roncella
    • 5
  • Robin Foà
    • 2
  • Guido Forni
    • 1
  1. 1.Istituto di MicrobiologiaUniversità degli Studi di TorinoTorinoItalia
  2. 2.Dipartimento di Scienze Biomediche ed Oncologia UmanaUniversità degli Studi di TorinoItalia
  3. 3.Servizio di Anatomia PatologicaOspedale Infantile Regina MargheritaTorino
  4. 4.Istituto di Patologia SperimentaleUniversità degli Studi di RomaItalia
  5. 5.Istituto Nazionale per la Ricerca sul CancroGenova

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