Research in Clinic and Laboratory

, Volume 19, Issue 1, pp 139–147 | Cite as

Immunocytochemical typing of fine needle aspirates by means of alkaline phosphatase anti-alkaline phosphatase method. A study of 16 selected patients

  • Pellegrino Musto
  • Michele Aieta
  • Giuseppe Sperandeo
  • Mario Cammisa
  • Mario Carotenuto
Original Contributions


The immunocytochemical characterization of neoplastic cells recognized by a large panel of monoclonal antibodies in fine needle aspirates obtained from 16 subjects with superficial or profound tumoral masses of unknown origin was carried out using alkaline phosphatase anti-alkaline phosphatase (APAAP) immunoenzymatic method. The patients were selected on the basis of particular clinical criteria or since a correct cytohistological diagnosis was not available. APAAP immunophenotyping allowed to establish the existence of non-Hodgkin lymphoma in 6 cases, hairy cell leukemia in 1 case and carcinoma in 5 cases, while in other 2 patients the existence of Hodgkin’s disease was suspected and subsequently confirmed; only in 2 subjects diagnostic informations were lacking. Furthermore, the evaluation of growth fraction by means of Ki 67 monoclonal antibody revealed a high degree of malignancy in all cases of lymphoma. Although some cautions have to be considered in the interpretation of results, APAAP immunocytochemical typing of fine needle aspirates appears a simple and reliable noninvasive diagnostic tool in selected patients.


APAAP method Fine needle aspirates Monoclonal antibodies Undifferentiated malignancies 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Pellegrino Musto
    • 1
  • Michele Aieta
    • 1
  • Giuseppe Sperandeo
    • 2
  • Mario Cammisa
    • 2
  • Mario Carotenuto
    • 1
  1. 1.Divisione di EmatologiaOspedale Generale Regionale «Casa Sollievo della Sofferenza’San Giovanni Rotondo (FG)Italia
  2. 2.Dipartimento di Diagnostica per ImmaginiOspedale Generale Regionale «Casa Sollievo della Sofferenza’San Giovanni Rotondo

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