Summary
The immunocytochemical characterization of neoplastic cells recognized by a large panel of monoclonal antibodies in fine needle aspirates obtained from 16 subjects with superficial or profound tumoral masses of unknown origin was carried out using alkaline phosphatase anti-alkaline phosphatase (APAAP) immunoenzymatic method. The patients were selected on the basis of particular clinical criteria or since a correct cytohistological diagnosis was not available. APAAP immunophenotyping allowed to establish the existence of non-Hodgkin lymphoma in 6 cases, hairy cell leukemia in 1 case and carcinoma in 5 cases, while in other 2 patients the existence of Hodgkin’s disease was suspected and subsequently confirmed; only in 2 subjects diagnostic informations were lacking. Furthermore, the evaluation of growth fraction by means of Ki 67 monoclonal antibody revealed a high degree of malignancy in all cases of lymphoma. Although some cautions have to be considered in the interpretation of results, APAAP immunocytochemical typing of fine needle aspirates appears a simple and reliable noninvasive diagnostic tool in selected patients.
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References
Anthoniuz P.: Value of immunocytochemical technics applied to products of cytopuncture in the cytodiagnosis of tumors and metastases — Arch. Anat. Cytol. Pathol.34, 99, 1986.
Chess Q., Hajdu S. I.: The role of immunoperoxidase staining in diagnostic cytology — Acta cytol. (Philad.)30, 1, 1986.
Coindre J. M., Tanguy F., Merlio J. P., De Mascarel I., De Mascarel A., Trojani M.: The value of immunohistological techniques in undifferentiated cancers — Tumori72, 539, 1986.
Coleman D. V., To A., Ormerod M. G., Dearnaley D. P.: Immunoperoxidase staining in tumor marker distribution studies in cytologic specimens — Acta cytol. (Philad.)25, 205, 1981.
Cordell J. L., Falini B., Erber W. N., Ghosh K. A., Abdulaziz Z., MacDonald S., Pulford K. A. F., Stein H., Mason D. Y.: Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes) — J. Histochem. Cytochem.32, 219, 1984.
Dalquen P.: Immunocytochemical cytodiagnostic of tumors — Verh. dtsch. Ges. Pathol.70, 387, 1986.
Delsol G., Gatter K. C., Stein H., Erber W. N., Pulford K. A. F., Zinne K., Mason D. Y.: Human lymphoid cells may express epithelial membrane antigen: implications for the diagnosis of human neoplasms — Lancetii, 1124, 1984.
Domagala W., Lubinski J., Weber K., Osborn M.: Intermediate filament typing of tumor cells in fine needle aspirates by means of monoclonal antibodies — Acta cytol. (Philad.)30, 214, 1986.
Domagala W., Weber K., Osborn M.: Differential diagnosis of lymph node aspirates by intermediate filament typing of tumor cells — Acta cytol. (Philad.)30, 225, 1986.
Droese M., Altmannsberger M., Kehl A., Lankish P. G., Weiss R., Weber K., Osborn M.: Ultrasound guided percutaneous fine needle aspirates biopsy of abdominal and retroperitoneal masses: accuracy of cytology in the diagnosis of malignancy, cytologic tumor typing and use of antibodies to intermediate filaments in selected cases — Acta cytol. (Philad.)28, 368, 1984.
Erber W. N., Mynheer L. C., Mason D. Y.: Labeling of blood and bone marrow samples for phenotyping leukemia — Lanceti, 761, 1986.
Falini B., Martelli M. F., Tarallo F., Moir D. J., Cordell J. L., Gatter K. C., Loreti G., Stein H., Mason D. Y.: Immunohistological analysis of human bone marrow trephine biopsies using monoclonal antibodies — Brit. J. Haematol.56, 365, 1984.
Falini B., Pileri S., Stein H., Rivano M. T., Minelli O., Fagioli M., Mori A., Martelli M. F.: Leucocyte antigen-negative malignant lymphomas. In: Int. Meeting on Genotypic, Phenotypic and Functional Aspects of Haematopoiesis. Assisi, April 6–8, 1987; p. 129. (Abstract).
Falini B., Pulford K. A. F., Erber W. N., Posnett D. N., Pallesen G., Schwarting R., Annino L., Cafolla A., Canino S., Mori A., Minelli O., Ciani C., Voxes E. E., Golomb H. M., Delsol G., Stein H., Martelli M. F., Grignani F., Mason D. Y.: Use of a panel of monoclonal antibodies for the diagnosis of hairy cell leukemia. An immunocytochemical study of 36 cases — Histopathology10, 671, 1986.
Ferrucci J. T., Wittenberg J., Mueller P. R., Simeone J. F., Harbin W. P., Kirkpatrick R. H., Taft P. D.: Diagnosis of abdominal malignancy by radiologic fine needle aspiration biopsy — Amer. J. Roentgenol.134, 323, 1980.
Gatter K. C., Alcock C., Heryet A., Mason D. Y.: Clinical importance of analysing malignant tumours of uncertain origin with immunohistological techniques — Lanceti, 1302, 1985.
Gerdes J., Dallenbach F., Lennert K.: Growth fraction in malignant non-Hodgkin’s lymphoma as determinedin situ with the monoclonal antibody Ki 67 — Hematol. Oncol.2, 365, 1984.
Gerdes J., Lemke H., Baisch H., Wacker H. H., Schwab U., Stein H.: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki 67 — J. Immunol.133, 1710, 1984.
Gustafsson B.: Methodological aspects and application of the immunoperoxidase staining technique in diagnostic fine needle aspirates cytology — Diagnost. Cytopathol.3, 68, 1987.
Heyderman E., Brown B. M. E.: Preparation of fine needle aspirates for immunocytochemical studies — Lancetii, 520, 1986.
Koss L. G., Woyke S., Oiszewsky W.: Aspiration Biopsy Cytologic Interpretation and Histological Bases. Igaku-Shoin, New York-Tokyo, 1984.
Linder J., Olsen G. A., Johnston W. W.: Fine needle aspiration biopsy of the mediastinum — Amer. J. Med.81, 1005, 1986.
Musto P., Bavaro P., Cascavilla N., Melillo L., Nobile M.: Tecniche immunoenzimatiche di caratterizzazione cellulare: particolari possibilità applicative nella citologia oncoematologica — Medicina (Rivista EMI)7, 38, 1987.
Pileri S., Gerdes J., Rivano M. T., Gobbi M., Taruscio D., Mazza P., Tura S., Stein H.: Linfoma istiocitico vero e linfomi a cellule attivate. In:Mandelli F., Tura S. (Eds): XII Corso di Aggiornamento in Ematologia Clinica. Esculapio, Bologna, 1986; p. 519.
Ramaekers F., Haag D., Jap P., Vooijs P. G.: Immunochemical demonstration of keratin and vimentin in cytologic aspirates — Acta cytol. (Philad.)28, 385, 1984.
Schwab U., Stein H., Gerdes J., Lemke H., Kirchner H., Schaadt M., Diehl V.: Production of a monoclonal antibody specific for Hodgkin and Reed-Sternberg cells of Hodgkin’s disease and a subset of normal lymphoid cells — Nature (Lond.)299, 265, 1982.
Selter D. M., Krajewsky A. S., Dewar A. E.: Immunohistochemical staining of non-Hodgkin’s lymphomas with monoclonal antibodies specific for the leucocyte common antigen — J. Pathol.146, 345, 1985.
Sloane J. P., Ormerod M. G.: Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology — Cancer (Philad.)47, 1586, 1981.
Tani E. M., Christensson B., Porwit A., Skoog L.: Immunocytochemical analysis and cytomorphologic diagnosis on fine needle aspirates of lymphoproliferative disease — Acta cytol. (Philad.)32, 209, 1988.
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Musto, P., Aieta, M., Sperandeo, G. et al. Immunocytochemical typing of fine needle aspirates by means of alkaline phosphatase anti-alkaline phosphatase method. A study of 16 selected patients. Res. Clin. Lab. 19, 139–147 (1989). https://doi.org/10.1007/BF02871802
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DOI: https://doi.org/10.1007/BF02871802