Abstract
Aim of the present study was to investigate on the possible alterations induced by on ground modeled microgravity on ion-water transport proteins at cellular level. For the purpose we used astrocytes, C6 line, neurons (NT2 line from human teratocarcinoma) and testicular cells (germ cells, Sertoli cells, and Leydig cells; primary cultures from trypsinised prepuberal pig testes). Modeled microgravity was achieved by a desktop 3D Random Positioning Machine, cultures were kept rotating for 30′, 1h and 24h. After 30′, immunopositivity for the antibodies to Na+/K+ATPase and Na+/K+/Cl− co-transporters was greatly diminished, the plasma membrane appeared to be altered, and the mitochondria inner cristae were disrupted. Immunostaining to the antibody to the water channel aquaporin 4 was very bright. After 1h at random rotation immunostaining for the heat shock protein Hsp27 was visible, After 24h, immunostaining for the ion transport proteins was again like that of the controls, plasma membrane and the mitochondria were again normal. Immunostaining for aquaporin 4 become again similar to that of the controls. We conclude that low gravity induces only transient alterations in the cell’s transmembrane ion-water transport: the cells are able to adapt to the gravity vector changes in few hours.
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References
Diana, N.: Transcapillary Water Flux. The Physiologists, vol. 25, pp. 365–375 (1970)
Leach, C.S., Alfrey C.P., Suki, W.N., Leonard, J.L., Rambaut, P.C., Inners, L.D., Smith, S.M., Lane, H.W., Krauhs, J.M.: Regulation of body fluid compartments during short-term space flight. J. Appl. Physiol., vol. 81, pp. 105–116 (1996)
Alfrey, C.P., Udden, M.M., Leach-Huntoon, C., Driscoll, T., Pickett, M.H.: Control of red blood cell mass in spaceflight. J. Appl. Physiol., vol.81, pp.98–104 (1996)
Uva, B.M., Masini, M.A., Sturla, M., Prato, P., Passalacqua, M., Giuliani, M., Tagliafierro, G., Strollo, F.: Clinorotation — induced weightlessness influences the cytoskeleton of glial cells in culture. Brain Res, vol. 934, pp. 132–139 (2002)
Shatten H, Lewis M, Chakraburti A.: Spaceflights and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells. Acta Astronautica, vol. 49, pp. 399–418. (2001)
Uva, B.M, Masini, M.A., Sturla M, Buzzone, F., Giuliani, M., Tagliaferro, G., Strollo, F.: Microgravity-induced apoptosis in cultured glial cells. Eur. J. Histochem., vol. 46, pp. 209–214. (2002)
Lang, F., Busch, G. L., Ritter, M., Volkl, H., Waldegger, S., Gulbins, E., Haussinger, D. Functional significance of cell volume regulatory mechanisms Physiol. Rev., vol 78, pp 247–306 (1998)
Haas, M. The Na+/K+/Cl− cotransporter. Am. J. Physiol., vol 267, pp C869-C885 (1994)
Platoshyn, O., Zhang, S., McDaniel S.S., Yuan JX. J.: Cytochrome — c activates K+ channels before inducing apoptosis. Am. J. Physiol. Cell. Physiol., vol. 283, pp C1289-C1305 (2002)
Paul, C., Manero, F., Gonin, S., Kretz-Remy C.,Virot, S., Arrigo, A.P.: Hsp 27 as a Negative Regulator of Cytochrome-c Release. Mol. Cell Biol., vol, 22, pp 816–834 (2002)
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Uva, B.M., Pastorino, M., Tagliafierro, G. et al. Ions and water transmembrane transport in nervous and testicular cultured cells in low gravity conditions. Microgravity Sci. Technol 18, 239–242 (2006). https://doi.org/10.1007/BF02870418
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DOI: https://doi.org/10.1007/BF02870418