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Ions and water transmembrane transport in nervous and testicular cultured cells in low gravity conditions

  • Bianca Maria Uva
  • Martina Pastorino
  • Grazia Tagliafierro
  • Maria Angela Masini
  • Felice Strollo
  • Franco Ricci
  • Maria Rita Mariani
Article

Abstract

Aim of the present study was to investigate on the possible alterations induced by on ground modeled microgravity on ion-water transport proteins at cellular level. For the purpose we used astrocytes, C6 line, neurons (NT2 line from human teratocarcinoma) and testicular cells (germ cells, Sertoli cells, and Leydig cells; primary cultures from trypsinised prepuberal pig testes). Modeled microgravity was achieved by a desktop 3D Random Positioning Machine, cultures were kept rotating for 30′, 1h and 24h. After 30′, immunopositivity for the antibodies to Na+/K+ATPase and Na+/K+/Cl co-transporters was greatly diminished, the plasma membrane appeared to be altered, and the mitochondria inner cristae were disrupted. Immunostaining to the antibody to the water channel aquaporin 4 was very bright. After 1h at random rotation immunostaining for the heat shock protein Hsp27 was visible, After 24h, immunostaining for the ion transport proteins was again like that of the controls, plasma membrane and the mitochondria were again normal. Immunostaining for aquaporin 4 become again similar to that of the controls. We conclude that low gravity induces only transient alterations in the cell’s transmembrane ion-water transport: the cells are able to adapt to the gravity vector changes in few hours.

Keywords

Leydig Cell Space Flight Simulated Microgravity Testicular Cell Water Channel Aquaporin 
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References

  1. 1.
    Diana, N.: Transcapillary Water Flux. The Physiologists, vol. 25, pp. 365–375 (1970)Google Scholar
  2. 2.
    Leach, C.S., Alfrey C.P., Suki, W.N., Leonard, J.L., Rambaut, P.C., Inners, L.D., Smith, S.M., Lane, H.W., Krauhs, J.M.: Regulation of body fluid compartments during short-term space flight. J. Appl. Physiol., vol. 81, pp. 105–116 (1996)Google Scholar
  3. 3.
    Alfrey, C.P., Udden, M.M., Leach-Huntoon, C., Driscoll, T., Pickett, M.H.: Control of red blood cell mass in spaceflight. J. Appl. Physiol., vol.81, pp.98–104 (1996)Google Scholar
  4. 4.
    Uva, B.M., Masini, M.A., Sturla, M., Prato, P., Passalacqua, M., Giuliani, M., Tagliafierro, G., Strollo, F.: Clinorotation — induced weightlessness influences the cytoskeleton of glial cells in culture. Brain Res, vol. 934, pp. 132–139 (2002)CrossRefGoogle Scholar
  5. 5.
    Shatten H, Lewis M, Chakraburti A.: Spaceflights and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells. Acta Astronautica, vol. 49, pp. 399–418. (2001)CrossRefGoogle Scholar
  6. 6.
    Uva, B.M, Masini, M.A., Sturla M, Buzzone, F., Giuliani, M., Tagliaferro, G., Strollo, F.: Microgravity-induced apoptosis in cultured glial cells. Eur. J. Histochem., vol. 46, pp. 209–214. (2002)Google Scholar
  7. 7.
    Lang, F., Busch, G. L., Ritter, M., Volkl, H., Waldegger, S., Gulbins, E., Haussinger, D. Functional significance of cell volume regulatory mechanisms Physiol. Rev., vol 78, pp 247–306 (1998)Google Scholar
  8. 8.
    Haas, M. The Na+/K+/Cl cotransporter. Am. J. Physiol., vol 267, pp C869-C885 (1994)Google Scholar
  9. 9.
    Platoshyn, O., Zhang, S., McDaniel S.S., Yuan JX. J.: Cytochrome — c activates K+ channels before inducing apoptosis. Am. J. Physiol. Cell. Physiol., vol. 283, pp C1289-C1305 (2002)Google Scholar
  10. 10.
    Paul, C., Manero, F., Gonin, S., Kretz-Remy C.,Virot, S., Arrigo, A.P.: Hsp 27 as a Negative Regulator of Cytochrome-c Release. Mol. Cell Biol., vol, 22, pp 816–834 (2002)CrossRefGoogle Scholar

Copyright information

© Z-Tec Publishing 2006

Authors and Affiliations

  • Bianca Maria Uva
    • 1
  • Martina Pastorino
    • 1
  • Grazia Tagliafierro
    • 1
  • Maria Angela Masini
    • 1
  • Felice Strollo
    • 2
  • Franco Ricci
    • 3
  • Maria Rita Mariani
    • 4
  1. 1.Dipartimento di BiologiaUniversità di GenovaItaly
  2. 2.Unità Endocrinologia INRCA & Università di Roma La SapienzaItaly
  3. 3.ENEA C.R.RomaItaly
  4. 4.ISTGenova

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